SeqVersa™ FS DNA Library Prep Kit (PCR-Free) enables high-quality DNA library preparation without the need for PCR amplification, preserving sequence integrity and reducing amplification bias.
SeqVersa™ FS DNA Library Prep Kit (PCR Free)
Price range: $796.00 through $3,006.00
Purchase this item and get Product Description
The SeqVersa™ FS DNA Library Prep Kit (PCR-Free) offers an optimized, user-friendly solution for preparing high-quality DNA libraries without PCR amplification. Designed for researchers seeking minimal GC bias and improved sequence representation, this kit incorporates an advanced enzymatic DNA fragmentation system that streamlines the workflow and reduces hands-on time.
With a broad input range (50 ng to 500 ng of intact DNA), SeqVersa™ FS ensures reliable fragmentation, efficient end repair, and dA-tailing using a single enzyme mix. The PCR-free protocol preserves native sequence complexity, making it ideal for applications requiring high accuracy and uniform coverage, such as whole-genome sequencing (WGS) and epigenetic studies.
Compatible with Illumina® sequencing platforms, the kit can be paired with SeqVersa™ multiplex index adapters or other Illumina-compatible adapters featuring a 3’ single T-overhang. An optional version with purification beads is also available for added convenience.
Kit Contents
● DNA Fragmentation Enzyme Mix
● End Repair Module
● Adapter Ligation Mix
● Sample Purification Beads
● User Manual
Technical Parameters
● Input DNA Amount: 250 ng to 2 µg
● Library Type: PCR-free DNA libraries
● Compatibility: Illumina sequencing platforms
● Protocol Time: Approximately 3-4 hours
Applications
● Whole-genome sequencing
● Structural variant detection
● Epigenomics
● High-fidelity sequencing of GC-rich and complex genomes
Product FAQs
What is SeqVersa™ FS DNA Library Prep Kit (PCR-Free) used for?
This kit is designed for PCR-free DNA library preparation, ideal for whole-genome sequencing and applications that require high sequence fidelity.
What input DNA amounts are suitable for this kit?
The kit is optimized for input DNA amounts ranging from 250 ng to 2 µg.
Why choose a PCR-free DNA library prep kit?
PCR-free library preparation reduces amplification bias, improves coverage uniformity, and enhances sequencing accuracy, especially for GC-rich regions.
Is SeqVersa™ FS DNA Library Prep Kit (PCR-Free) compatible with Illumina sequencing?
Yes, the kit is fully compatible with Illumina sequencing platforms, ensuring high-quality sequencing output.
What are the advantages of using SeqVersa™ FS DNA Library Prep Kit (PCR-Free)?
This kit eliminates PCR-related biases, preserves original DNA complexity, and enhances sequencing accuracy, making it ideal for applications that require high-fidelity data.
| Kit Capacity | 24 reactions, 96 reactions |
|---|
Related products
-
SeqVersa™ Poly-A mRNA Isolation Kit
Price range: $656.00 through $2,495.00The SeqVersa™ Poly-A mRNA Isolation Kit offers a highly efficient method for isolating polyadenylated mRNA from total RNA, ensuring high purity and yield. This kit is ideal for preparing mRNA samples for downstream applications such as next-generation sequencing (NGS) and quantitative PCR (qPCR).Select options This product has multiple variants. The options may be chosen on the product page Show more -
SeqVersa™ Directional RNA Library Prep Kit
Price range: $1,081.00 through $3,672.00SeqVersa™ Directional RNA Library Prep Kit enables robust and efficient preparation of directional RNA libraries for Illumina sequencing, ensuring accurate strand-specific RNA analysis.Select options This product has multiple variants. The options may be chosen on the product page Show more -
SeqVersa™ RNA First Strand Synthesis Kit
Price range: $174.00 through $555.00The SeqVersa™ RNA First Strand Synthesis Kit provides a reliable and efficient solution for synthesizing first-strand cDNA from RNA templates. Designed for use in next-generation sequencing and other molecular biology applications, it ensures high yield and reproducibility.Select options This product has multiple variants. The options may be chosen on the product page Show more -
SeqVersa™ Fragmented FFPE DNA Library Prep Kit
Price range: $860.00 through $3,270.00The SeqVersa™ Fragmented FFPE DNA Library Prep Kit for Illumina offers a comprehensive solution for high-quality DNA fragmentation and library preparation. Utilizing a proprietary enzymatic shearing technology, this kit ensures precise and consistent fragment sizes, making it ideal for next-generation sequencing applications.Select options This product has multiple variants. The options may be chosen on the product page Show more
Updating…