Detect pathogen nucleic acids with ease using the OptiSense™ Pathogen +IC Kit (100), featuring preoptimized internal controls for reliable multiplex real-time PCR, a unique two-phase hot-start procedure for convenient setup, and visual pipetting control for enhanced accuracy.
OptiSense™ Pathogen +IC Kit
Price range: $226.00 through $1,834.00
Purchase this item and get Product Description
The OptiSense™ Pathogen +IC Kit (100) is an advanced molecular biology tool designed for precise and sensitive detection of pathogen nucleic acids. Utilizing state-of-the-art technology, this kit enables rapid and accurate identification of a wide range of pathogen targets, including viruses, bacteria, and fungi. Its innovative design incorporates preoptimized internal controls, ensuring the reliability and reproducibility of results across multiple assays.
Additionally, the kit features a unique two-phase hot-start procedure, which enhances reaction specificity and minimizes non-specific amplification, even during complex multiplex assays. This advanced hot-start mechanism provides exceptional stability during reaction setup, allowing for seamless automation and streamlined laboratory workflows.
Furthermore, the OptiSense™ Pathogen +IC Kit offers a comprehensive solution for real-time PCR detection, with reagents specifically formulated to deliver robust performance over a wide linear range. The inclusion of visual pipetting control further enhances assay accuracy, enabling precise sample handling and minimizing errors. With its ultrafast, ready-to-use format, this kit simplifies experimental procedures and accelerates time-to-results, making it an ideal choice for high-throughput screening and diagnostic applications.
Kit Contents
● OptiSense™ Pathogen Master Mix (1 x 0.5 mL)
● Yellow Template Dilution Buffer (1 x 0.5 mL)
● ROX Reference Dye (1 x 0.25 mL)
● Nuclease-Free Water (1 x 1.9 μl)
● Nucleic Acid Dilution Buffer (1 x 1500 μl)
● Internal Control RNA (1 x 100 μl)
● Internal Control DNA (1 x 100 μl)
● IC Probe Assay (1 x 200 μl)
Technical Parameters
● Reactions: 100 x 20 μl
● Linear Range: Wide linear range without loss of sensitivity when multiplexing
● Storage: Master mix can be stored at 2–8°C for up to 12 months
Applications
Sensitive real-time PCR or one-step RT-PCR for simultaneous detection of pathogen DNA and/or RNA, including an internal control.
Product FAQs
What is the purpose of the internal controls included in the kit?
The internal controls serve as extraction or amplification controls, ensuring the correct interpretation of negative detection results by monitoring the efficiency of the purification process and the PCR or RT-PCR amplification.
How does the unique two-phase hot-start procedure benefit the reaction setup?
The two-phase hot-start procedure allows for manual or automated reaction setup at room temperature without compromising performance, ensuring stable RT and increasing overall workflow efficiency.
Can the kit be used for multiplexing?
Yes, the kit enables 4-plex (RT)-PCR for the detection of multiple pathogen targets simultaneously without loss of sensitivity.
What is the shelf life of the master mix?
The master mix can be stored at 2–8°C for up to 12 months, providing long-term convenience and reliability.
Is the kit suitable for automated workflows?
Yes, the kit allows for automated reaction setup at room temperature, streamlining laboratory processes and enhancing efficiency.
| Kit / Assay type | Pathogen +IC Kit, IC Assay Kit |
|---|---|
| Reactions | 100, 200, 500 |
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