FusionTaq™ MasterMix Kit is an instant upgrade to your PCR setup with a easy-to-use premix of all components. The kit guarantees a quick, simplified and efficient output for your PCR experiments without the need of extra optimization and at room temprature. The MasterMix format ensures a reproducible, high-throughput and contamination free alternative. The procedure stands out for its exceptional speed and simplicity, offering maximum convenience to users. Its streamlined approach ensures efficient amplification processes, making it remarkably user-friendly and time-effective.
FusionTaq™ MasterMix Kit
Price range: $300.00 through $2,474.00
Purchase this item and get Product Description
FusionTaq™ MasterMix kit comes pre-packaged with the groundbreaking FusionTaq™ DNA polymerase, OptiMix™ buffer and dNTPs, all in a single, user-friendly tube to make all PCR setups a breeze. The FusionTaq Polymerase by itself features no activity at low and ambient temperatures and is only active after a hot-start procedure prior to the PCR thermocycling. The polymerase is accompanied by the innovative AvBlend™, an integral component within the kit designed for next-generation dual-cation assistance in amplifying complex templates. Its primary function lies in fine-tuning the DNA melting behavior, thereby facilitating the amplification of templates that present inherent challenges due to their GC-rich nature.
The OptiMix PCR buffer, a remarkable inclusion, plays a pivotal role in altering primer binding efficiencies. This adjustment ensures the attainment of high specific to non-specific primer binding ratios, thus guaranteeing the highly specific amplification of the target DNA. Moreover, the buffer is formulated with a pre-optimized quantity of Mg+, eliminating the necessity for experimental optimization during the reaction setup. Consequently, it streamlines the PCR procedure, rendering it more efficient and user-friendly.
Furthermore, the polymerase itself not only assures rapid and precise results but also boasts a high unitage, allowing for multiple reactions to be conducted. This advantageous feature is offered at a competitive price point, enhancing its appeal and accessibility within various research settings.
Kit Contents
For 250U
FusionTaq™ Master Mix (3 X 0.85 mL, contains a total of 250 units of FusionTaq™ DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 μM of dNTPs)
Nuclease-Free Water (2 X 1.7 mL)
Technical Parameters
Incubation for FusionTaq™ hotstart: 95°C for 15 minutes
5’-3’ exonuclease acitivity
1000000 Fold amplification efficiency with a high specific to non-specific primer binding ratio.
Polymerase extends at 2-4 kb/minute at 72°C
Polymerase has the capability to add extra As.
Applications
● Reactions involving two or more primer pairs
● Genomic templates
● cDNA templates
● Low-copy transcript targets
Product FAQs
How is the primer annealing enhanced with this kit?
The kit components promote a high specific to non-specific primer binding ratio thanks to ionic concentrations allowing easy stabilization, destablilization and restabilization of bonds formed during the annealing process.
Do I need a separate thermocycler for the hot-start step?
No, the hot-start step can seamlessly integrate into any existing thermocycler program thus making it a convenient process.
Can this improve my less-than-optimal PCR reactions?
The use of FusionTaq™ Polymerase and its other accompaniments ensures a highly optimal and streamlined output to any existing PCR setups with less-than-optimal results.
Does this kit have any multiplex applications?
The kit can be used for multiple primer pairs and due to its high specific primer binding capability is capableto be utilized in multiplex PCR reactions.
| Quantity | 1000U, 2500U, 250U |
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