The OptiClean™ PCR Kits is a revolutionary solution designed to meet the demanding requirements of molecular biology applications, offering exceptional sensitivity, specificity, and reliability in real-time PCR analyses.
OptiClean™ PCR Kits
Price range: $170.00 through $693.00
Purchase this item and get Product Description
The OptiClean™ PCR Kits represents the forefront of real-time PCR technology, providing researchers with an advanced toolkit to achieve accurate and reproducible results across a wide range of applications. Engineered with precision and innovation, this kit sets a new standard in nucleic acid analysis, delivering unparalleled performance and convenience.
At the heart of the OptiClean™ PCR Kits is a meticulously crafted master mix formulation, meticulously purified to eliminate background contamination and ensure the highest levels of accuracy. The inclusion of nucleic acid-depleted reagents ensures that only the target DNA or cDNA is amplified, minimizing the risk of false positives and guaranteeing the integrity of your results. Moreover, the OptiClean™ PCR Kits features a unique hot-start mechanism, leveraging antibody-mediated inhibition to prevent non-specific amplification and primer-dimer formation. This stringent hot-start procedure ensures superior specificity and sensitivity, allowing for the detection of even the lowest-abundance transcripts with confidence.
Designed for ease of use and maximum efficiency, the OptiClean™ PCR Kits streamlines your workflow with its visual pipetting control mechanism, enabling error-free reaction setup. Its room-temperature reaction setup and ultrafast cycling protocols further enhance productivity, allowing you to obtain results in less time without compromising on quality.
Kit Contents
Probe Kit
● 2X OptiClean™ Probe PCR MasterMix (5 X 1 mL)
● OptiClean™ Yellow Template Dilution Buffer (1 x 0.2 mL)
● OptiClean™ ROX Dye (2 x 0.25 mL)
● OptiClean™ PCR Water (4 x 1.9 mL)
SYBR Green Kit
● OptiClean™ SYBR Green PCR MasterMix (5 x 1 mL)
● OptiClean™ Yellow Template Dilution Buffer (1 x 0.2 mL)
● OptiClean™ ROX Dye (2 X 0.25 mL)
● OptiClean™ PCR Water (4 x 1.9 mL)
Technical Parameters
Detection Type: Probe, SYBR Green Room-temperature reaction setup Integrated visual pipetting control Ultrafast two-step cycling protocols.
Applications
Microbial testing
Microbiome/metagenome analysis
Quality control procedures
Quantitative nucleic acid analyses
Detection and quantification of contaminating or residual DNA
Product FAQs
How does the OptiClean™ PCR Kits ensure specificity in amplification?
The kit utilizes a unique hot-start mechanism, where the DNA polymerase remains inactive until the initial heat activation step. This stringent control prevents non-specific amplification and primer-dimer formation, ensuring high specificity in amplification.
Can the OptiClean™ PCR Kits be used for quantification of low-abundance transcripts?
Yes, the kit offers exceptional sensitivity and quantification over a wide linear range, making it suitable for detecting even the lowest-abundance transcripts with high precision and accuracy.
Is the OptiClean™ PCR Kits compatible with high-throughput applications?
Yes, the kit is designed for easy parallel processing of several samples and can be seamlessly integrated into high-throughput workflows, allowing for rapid analysis of multiple samples with consistency and reliability.
How does the OptiClean™ PCR Kits minimize background contamination?
The kit incorporates nucleic acid-depleted reagents, which are rigorously purified to eliminate background contamination from residual DNA. This ensures the integrity of your results and minimizes the risk of false positives.
Can the OptiClean™ PCR Kits be used for microbial community analysis?
Yes, the kit is highly recommended for microbial testing and microbiome/metagenome analysis, offering superior performance in quantifying microbial DNA and facilitating comprehensive analysis of microbial communities.
What is the advantage of the visual pipetting control mechanism?
The visual pipetting control mechanism provides a convenient way to ensure accurate reaction setup, minimizing pipetting errors and enhancing the reliability of your results. The color change indicates proper pipetting, enhancing user confidence in the experimental setup.
| Detection types | Probe, SYBR Green |
|---|---|
| Reactions | 100, 500 |
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