OptiScript™ PCR Kits

OptiScript™ PCR Kits

Price range: $615.00 through $4,024.00

Achieve highly sensitive and accurate quantification of genomic DNA and cDNA targets with the OptiScript™ PCR Kit (Probe). This kit offers the convenience of real-time PCR using sequence-specific probes, enabling precise quantification without the need for extensive optimization.

Purchase this item and get 61-402 Points
Purchase this item and get 61-402 Points
Product Description

The OptiScript™ PCR Kits are state-of-the-art solution for researchers requiring robust and reproducible quantification of nucleic acid targets. This advanced kit leverages the power of real-time PCR technology combined with sequence-specific probes to deliver exceptional performance across a wide range of applications.

At the heart of this kit is the proprietary OptiScript™ PCR Master Mix, meticulously formulated to provide optimal conditions for PCR amplification. This master mix contains FusionTaq™ DNA Polymerase, known for its stringent hot start feature, which prevents nonspecific amplification and ensures reliable results. Additionally, the inclusion of a unique PCR buffer system promotes specific primer annealing, enhancing PCR specificity and sensitivity.

The OptiScript™ PCR Kits are characterized by its high sensitivity, capable of detecting low-copy targets with exceptional accuracy. The kit offers accurate quantification over a wide linear range, enabling precise measurement of target concentrations spanning several orders of magnitude. Researchers can confidently quantify up to 5 targets in the same tube, streamlining experimental workflows and conserving precious samples.

One of the key advantages of the OptiScript™ PCR Kits are its minimal optimization requirement. The optimized master mix minimizes the need for extensive optimization of reaction and cycling conditions, saving researchers valuable time and resources. Furthermore, the kit’s dNTP mix includes dUTP, allowing optional treatment with uracil-N-glycosylase (UNG) to eliminate carryover contamination, ensuring data integrity.

For added convenience, the master mix can be stored at 2–8°C for extended periods without compromising performance, providing flexibility in experimental planning and execution. Whether performing gene expression analysis, pathogen detection, or molecular diagnostics, the OptiScript™ PCR Kits offer unparalleled sensitivity, accuracy, and ease of use.

Kit Contents

For 200 reactions in the Probe Kit:
2X OptiScript™ Probe PCR MasterMix (3 X 1.7 mL)
Nuclease-Free Water (2 x 2 mL)

For 200 reactions in the SYBR Green Kit:
2X OptiScript™ SYBR Green PCR MasterMix (3 X 1.7 mL)
Nuclease-Free Water (2 X 2 mL)

For 200 reactions in the Multiplex Probe Kit:
2X OptiScript™ Multiplex PCR NoROX MasterMix (without ROX dye) or ROX MasterMix (3 X 1.7 mL)
Nuclease-Free Water (2 X 2 mL)

For 200 reactions in the Probe Kit:
2X OptiScript™ Probe PCR MasterMix (3 X 1.7 mL)
Nuclease-Free Water (2 x 2 mL)

For 200 reactions in the Probe+UNG Kit:

2X OptiScript™ Probe PCR MasterMix (3 X 1.7 mL) UNG Solution (1 X 0.1mL)
Nuclease-Free Water (2 X 2 mL)

For 200 reactions in the SYBR Green+UNG Kit:
2X OptiScript™ SYBR Green PCR MasterMix (3 X 1.7 mL) UNG Solution (1 X 0.1mL)
Nuclease-Free Water (2 X 2 mL)

Technical Parameters

●  Concentration: 5 units/μl
●  Recombinant Enzyme: Yes
●  Substrate Analogues: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTP
●  Extension Rate: 2–4 kb/min at 72°C
●  Half-life: 10 min at 97°C; 60 min at 94°C
●  Amplification Efficiency: ≥10^5 fold
●  5’–>3′ Exonuclease Activity: Yes
●  Extra A Addition: Yes
●  3’–>5′ Exonuclease Activity: No
●  Contaminating Nucleases: No
●  Contaminating RNases: No
●  Contaminating Proteases: No
●  Self-priming Activity: No

Applications

The OptiScript™ PCR Kit is suitable for various molecular biology applications, including:

●  Real-time quantification of genomic DNA or cDNA targets
●  Two-step RT-PCR
●  Multiplex PCR

Product FAQs
Can this kit be used for multiplex PCR?

Yes, the OptiScript™ PCR Kit (Probe) allows detection of up to 5 targets in the same tube, facilitating multiplex PCR experiments.

How long can the master mix be stored?

The master mix can be stored at 2–8°C for extended periods, providing long-term convenience and stability for experimental setup.

Is optimization required for real-time PCR setup?

Minimal optimization is necessary due to the kit’s optimized master mix, reducing the need for extensive optimization of reaction conditions.

What is the advantage of including dUTP in the dNTP mix?

The inclusion of dUTP enables optional treatment with uracil-N-glycosylase (UNG), effectively eliminating carryover contamination from previous PCR reactions and ensuring data accuracy.

Can this kit be used for pathogen detection?

Yes, the OptiScript™ PCR Kit (Probe) is suitable for pathogen detection applications, offering high sensitivity and accurate quantification for reliable results.

Kit Type

OptiScript PCR Kit, OptiScript PCR + UNG Kit
Detection Type

Probe, Multiplex Probe, SYBR Green
Reference Dye

ROX, No ROX, N/A
Reactions

200, 1000, N/A
SKU: PK4730 Category:
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