Avam® RNase Inhibitor (Human Source) effectively safeguards RNA integrity by inhibiting RNase A, RNase B, and RNase C activities, ensuring reliable results in molecular biology experiments.
Avam® RNase Inhibitor (Human source)
Price range: $76.00 through $322.00
Purchase this item and get Product Description
Avam® RNase Inhibitor (Human Source) is a vital tool for maintaining RNA integrity in various molecular biology applications. This inhibitor, derived from a thermally resistant, recombinant human placental protein expressed in Escherichia coli, offers potent inhibition against common eukaryotic RNases, including RNase A, RNase B, and RNase C. Supplied in a solution containing 20 mM Hepes-KOH (pH 7.6), 50 mM KCl, 8 mM reducing agent, and 50% (v/v) glycerol, it remains stable even under challenging conditions, ensuring reliable protection of RNA samples. Each unit of the inhibitor effectively neutralizes the activity of 5 ng of RNase A, guaranteeing high-quality RNA suitable for downstream applications such as RNA-related molecular diagnostics, cDNA synthesis, RT-PCR, RT-qPCR, in vitro transcription and translation, and preparation of RNase-free monoclonal antibodies.
Kit Contents
Avam® RNase Inhibitor Solution (Human Source)
Technical Parameters
● Purity: >90%
● RNase contamination: None detected
● Latent RNase contamination: None detected
● Exonuclease contamination: None detected
● Endonuclease contamination: None detected
Applications
Avam® RNase Inhibitor (Human Source) is indispensable for overcoming challenges associated with ubiquitous RNases found in various sources, including skin, dust, reagents, and biological samples. Its applications include RNA-related molecular diagnostics, RNA isolation and purification, cDNA synthesis, RT-PCR, RT-qPCR, in vitro transcription and translation, and RNase-free monoclonal antibody preparation.
Product FAQs
What is the unit definition of Avam® RNase Inhibitor (Human Source)?
Each unit is defined as the amount of enzyme required to inhibit the activity of 5 ng RNase A by 50%.
How should I store the Avam® RNase Inhibitor (Human source)?
It is recommended to store the inhibitor solution at –25°C to –15°C for optimal stability.
Can Avam® RNase Inhibitor (Human Source) be used with other enzymes?
Yes, it does not influence the activity of polymerases or reverse transcriptases and remains compatible with various reagents and enzymes commonly used in molecular biology applications.
| Quantity | 10000U, 2000U |
|---|
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