Ni-NTA Spin Column Kit

Ni-NTA Spin Column Kit

Price range: $633.00 through $737.00

Rapid and efficient purification of His-tagged proteins with ready-to-use Ni-NTA spin columns.

Purchase this item and get 63-73 Points
Purchase this item and get 63-73 Points
Product Description

The Ni-NTA Spin Kit offers a convenient solution for the purification of His-tagged proteins in a microspin format. Each spin column contains Ni-NTA silica, allowing for high-affinity binding of His-tagged proteins from cleared cell lysates. The Ni-NTA Spin Kit utilizes Ni-NTA silica, which combines Ni-NTA with a macroporous silica support material optimized to suppress nonspecific hydrophobic interactions. The patented Ni-NTA resin provides high-affinity binding of His-tagged proteins, allowing for one-step purification under native or denaturing conditions. This technology enables rapid automated or manual processing of multiple samples in parallel. This kit enables purification of up to 300 µg of protein per column in as little as 15 minutes, with options for both native and denaturing conditions. The ready-to-use spin columns provide a simple method for functional screening of engineered proteins, selection of clones expressing full-length translation products, and comparison of expression levels.

Kit Contents

● 50 Ni-NTA Spin Columns
● Reagents
● Buffers
● Collection Tubes
● 1 μg Control Expression Plasmid

Technical Parameters​

● 50 Ni-NTA Spin Columns
● Reagents
● Buffers
● Collection Tubes
● 1 μg Control Expression Plasmid

Applications

● Structural and functional investigations
● Crystallization for determination of three-dimensional structure
● Assays involving protein–protein and protein–DNA interaction
● Immunization to produce antibodies

Product FAQs
What is the binding capacity of each Ni-NTA Spin Column?

Each Ni-NTA Spin Column can purify up to 300 µg of His-tagged protein.

Can the Ni-NTA Spin Kit be used for both native and denaturing purification conditions?

Yes, the kit supports purification under both native and denaturing conditions, offering flexibility in purification protocols.

What is the processing scale of the Ni-NTA Spin Kit?

The kit is designed for small-scale processing, suitable for low-throughput screening and rapid purification of His-tagged proteins.

Are the Ni-NTA Spin Columns compatible with automated procedures?

Yes, the Ni-NTA Spin Columns can be used for automated processing, providing reproducible and efficient purification results.

Quantity

Spin Columns: 50 columns, Spin Kit: 50 columns
SKU: PA3578 Category:
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