Ni-NTA Curio™ Kit

$491.00

The Ni-NTA Curio™ Kit offers a streamlined solution for efficient purification and detection of His-tagged proteins, ideal for beginners in protein science, providing prefilled Ni-NTA columns, antibodies, buffers, and straightforward protocols.

Purchase this item and get 49 Points
Purchase this item and get 49 Points
Product Description

The Ni-NTA Curio™ Kit is more than just a collection of reagents; it represents a comprehensive solution for researchers new to protein science, offering a seamless path towards efficient purification and detection of His-tagged proteins. Engineered to simplify and expedite the purification process, this kit equips users with all necessary components, from prefilled Ni-NTA columns to detection antibodies, eliminating the need for piecemeal sourcing and protocol development.

With a focus on accessibility and user-friendliness, the Ni-NTA Curio™ Kit features easy-to-follow protocols and straightforward processing steps. minimizing the learning curve for those unfamiliar with protein purification procedures. Whether performing purifications under native or denaturing conditions, the kit’s versatile buffers accommodate various experimental needs, ensuring reliable results across diverse applications.

The inclusion of an Anti-His antibody enhances the utility of the kit, enabling convenient detection of expressed His-tagged proteins following purification. This integrated approach streamlines workflows and reduces the time and effort required for both purification and subsequent analysis.

Backed by the remarkable selectivity of patented Ni-NTA resin, the kit enables one-step purification of His-tagged proteins with high affinity, yielding protein preparations of exceptional purity. The advanced Ni-NTA technology, with its superior chelation properties and minimized nickel-ion leaching, ensures optimal purification efficiency and enhances the yield and quality of purified proteins compared to traditional methods.

Furthermore, the Ni-NTA Curio™ Kit caters to a broad range of applications, from functional studies to structural investigations, offering researchers the flexibility to explore diverse avenues in protein research. Whether embarking on immunization studies, analyzing protein-protein interactions, or delving into proteomic analyses, users can rely on the kit’s performance and versatility to deliver consistent and reproducible results.

In summary, the Ni-NTA Curio™ Kit represents a turnkey solution for newcomers and experienced researchers alike, offering a convenient and efficient means to embark on protein purification endeavors. By consolidating essential components and simplifying protocols, the kit empowers users to delve into the fascinating realm of protein science with confidence and ease.

Kit Contents

● 6 x Curio™ Columns
● Penta·His Antibody
● Buffers and Reagents

Technical Parameters​

● Applications: Proteomics
● Support/matrix: Ni-NTA matrix
● Processing: Manual
● Binding capacity: 5–20 mg/ml
● Special feature: Ni-NTA technology
● Gravity flow or spin column: Gravity flow
● Start material: Cell lysate
● Tag: 6xHis tag
● Yield: <10 mg protein per column

Applications

● Functional studies
● Antibody production via immunization
● Assays involving protein-protein and protein-DNA interactions
● Structural investigations

Product FAQs
Can this kit be used for purification of His-tagged proteins from sources other than E. coli lysates?

While optimized for E. coli lysates, the Ni-NTA Curio™ Kit can potentially be adapted for use with lysates from other sources with proper optimization and validation.

How long does the purification process take with the Ni-NTA Curio™ Kit?

Purification of His-tagged proteins can be achieved in as little as 90 minutes using this kit, offering a rapid and efficient workflow.

Is it possible to elute His-tagged proteins under native conditions only?

No, the kit offers flexibility for elution under both native and denaturing conditions, providing options based on the experimental requirements

What is the recommended storage condition for the Ni-NTA Curio™ Kit?

It is recommended to store the kit components according to the instructions provided in the manual, typically at temperatures ranging from 2-8°C to maintain stability and functionality.

Quantity

6 preps

SKU: PA8720 Category:
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