Ni-NTA Agarose

Ni-NTA Agarose

Price range: $435.00 through $6,488.00

High-affinity resin for efficient purification of His-tagged proteins with options for native or denaturing conditions.

Purchase this item and get 43-648 Points
Purchase this item and get 43-648 Points
Product Description

The Ni-NTA Agarose utilizes patented Ni-NTA resin, which demonstrates high selectivity for proteins containing a His tag comprising six or more histidine residues. This technology allows for one-step purification of His-tagged proteins from various expression systems, including bacteria, yeast, mammalian cells, and baculovirus. The resin’s design prevents nickel-ion leaching, resulting in higher purity protein preparations compared to other metal-chelating purification systems. Ni-NTA Agarose is a robust affinity chromatography matrix designed for the purification of recombinant proteins carrying a His tag. Histidine residues within the His tag selectively bind to immobilized nickel ions on the resin with high specificity and affinity. This one-step purification system offers >95% pure protein yield from cleared cell lysates and is suitable for both native and denaturing purification conditions. The resin is precharged and ready to use, enabling automated purification and assay protocols for any scale of purification.

Kit Contents

Nickel-charged resin

Technical Parameters​

● Bead size: 45–165 µm
● Binding capacity: Up to 50 mg/mL
● Gravity flow or spin column: Gravity flow
● Yield: 100 µg – 100 mg
● Max. pressure: 2.8 psi

Applications

● Structural and functional investigations
● Crystallization for determining three-dimensional structure
● Protein-protein and protein-DNA interaction assays
● Immunization for antibody production
● Scale-up purification to production scale

Product FAQs
What is the maximum pressure tolerance for the Ni-NTA Agarose resin?

The Ni-NTA Agarose resin can withstand a maximum pressure of 2.8 psi during purification procedures.

Can the Ni-NTA Agarose resin be used for both native and denaturing purification conditions?

Yes, the resin is compatible with both native and denaturing conditions, offering flexibility in purification protocols.

What is the binding capacity of the Ni-NTA Agarose resin?

The resin has a binding capacity of up to 50 mg/ml, allowing for efficient purification of His-tagged proteins.

What expression systems are compatible with the Ni-NTA Agarose resin?

The Ni-NTA Agarose resin is compatible with various expression systems, including bacteria, yeast, mammalian cells, and baculovirus, for the purification of His-tagged proteins.

Quantity

100ml, 500ml, 25mL
SKU: PA4912 Category:
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