The SeqVersa™ Small RNA Multiplex Library Prep Kit offers comprehensive solutions for high-throughput small RNA sequencing on Illumina platforms, featuring versatile sets for indexing and efficient library preparation.
SeqVersa™ Small RNA Multiplex Library Prep Kit
Price range: $1,676.00 through $5,699.00
Purchase this item and get Product Description
The SeqVersa™ Small RNA Multiplex Library Prep Kit is designed to streamline small RNA sequencing workflows, providing researchers with robust tools for creating multiplexed libraries on Illumina platforms. This kit integrates three versatile sets (Index Primers 1-48, Set 1, and Set 2), each optimized for different sequencing needs and sample types.
Index Primers 1-48:
The Index Primers 1-48 set provides a wide range of indexing options, enabling precise sample identification and multiplexing efficiency in small RNA sequencing experiments.
Set 1:
Set 1 is tailored for specific small RNA library preparation needs, ensuring high sensitivity and accuracy in sequencing data for various biological applications.
Set 2:
Set 2 enhances flexibility with additional indexing capabilities, allowing researchers to expand multiplexing capacity while maintaining sequencing quality and performance.
The SeqVersa™ kit employs NEBNext® technology for reliable and reproducible library preparation, minimizing bias and optimizing small RNA capture across a broad size range. It includes reagents for adapter ligation, reverse transcription, PCR amplification, and RNA size selection, facilitating straightforward integration into existing workflows.
With an emphasis on data quality and sample throughput, SeqVersa™ enables researchers to explore complex small RNA populations with confidence, supporting applications such as miRNA profiling, non-coding RNA analysis, and biomarker discovery.
Kit Contents
● Index Primers 1-48
● Set 1 Library Prep Components
● Set 2 Library Prep Components
● Ligation Enzyme Mix
● PCR Master Mix
● RNA Size Selection Beads
Technical Parameters
● Index Options: 48 index primers (Index Primers 1-48)
● Compatibility: Illumina sequencing platforms
● Applications: Small RNA sequencing, miRNA profiling, non-coding
RNA analysis
● Storage Conditions: -20°C for all reagents
Applications
● Small RNA Sequencing
● miRNA Profiling
● Non-coding RNA Analysis
● Biomarker Discovery
Product FAQs
What is the advantage of using SeqVersa™ Small RNA Multiplex Library Prep Kit?
SeqVersa™ offers comprehensive solutions with multiple indexing options (Index Primers 1-48, Set 1, Set 2), enhancing flexibility and accuracy in small RNA sequencing experiments.
How does SeqVersa™ ensure reliable small RNA library preparation?
The kit integrates SeqVersa™ technology for optimized adapter ligation, reverse transcription, and PCR amplification, ensuring high reproducibility and minimal bias in small RNA capture.
Can SeqVersa™ be used for miRNA profiling and biomarker discovery?
Yes, SeqVersa™ is suitable for various applications including miRNA profiling and biomarker discovery, providing researchers with robust tools for small RNA analysis.
Are there specific storage requirements for SeqVersa™ components?
All components should be stored at -20°C to maintain stability and performance, as indicated in the user manual provided with the kit.
How many samples can be multiplexed using SeqVersa™?
SeqVersa™ supports efficient multiplexing of small RNA libraries, accommodating diverse experimental needs and enhancing sample throughput in sequencing studies.
Is there a recommended protocol for RNA size selection using SeqVersa™?
Yes, the user manual includes detailed protocols for RNA size selection using the provided RNA size selection beads, ensuring optimal library preparation for small RNA sequencing.
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