SeqVersa™ RNA Library Prep Kit

Price range: $1,220.00 through $4,610.00

The SeqVersa™ RNA Library Prep Kit for Illumina enables the preparation of high-quality RNA libraries for next-generation sequencing with a streamlined, fast, and efficient workflow.

Purchase this item and get 122-461 Points
Purchase this item and get 122-461 Points
Product Description

The SeqVersa™ RNA Library Prep Kit for Illumina offers a streamlined, highly efficient solution for RNA library preparation, specifically designed to support a broad range of RNA inputs from as low as 10 ng up to 1 µg. This kit integrates multiple steps, including RNA fragmentation, reverse transcription, end repair, adaptor ligation, and PCR enrichment into a single, cohesive workflow, drastically reducing hands-on time and potential sample loss. The kit’s reagents and protocols have been optimized to ensure maximum yield, high-quality libraries, and consistent results, even with challenging samples. The innovative design of the SeqVersa™ RNA Library Prep Kit includes robust enzymes and buffers that enhance the efficiency and effectiveness of each reaction step. This results in superior transcript representation and minimized bias, which are critical for applications such as mRNA and total RNA sequencing, gene expression profiling, and transcriptome analysis. The process, taking approximately 3.5 hours, is both fast and reliable, ensuring that researchers can move quickly from RNA to high-quality sequencing-ready libraries.

Kit Contents

● SeqVersa™ FS Enzyme Mix
● SeqVersa™ FS Reaction Buffer
● SeqVersa™ Ligation Master Mix
● SeqVersa™ Adaptor
● SeqVersa™ End Repair/dA-Tailing Module
● SeqVersa™ Reverse Transcriptase
● SeqVersa™ Primer Mix
● SeqVersa™ PCR Master Mix

Technical Parameters​

● Input RNA Amount: 10 ng to 1 µg
● Total Time: ~3.5 hours
● Storage: -20°C
● Enzyme Mix Volume: 50 µl
● Compatible Platforms: Illumina

Applications

● mRNA sequencing
● Total RNA sequencing
● Gene expression profiling
● Transcriptome analysis
● Clinical research

Product FAQs
What is the input RNA range for this kit?

The kit supports RNA inputs from 10 ng to 1 μg.

Is the kit compatible with both mRNA and total RNA?

Yes, it is suitable for both mRNA and total RNA sequencing applications.

What sequencing platforms are supported?

The libraries prepared are compatible with Illumina sequencing platforms.

How long does the library preparation process take?

The entire process takes approximately 3.5 hours.

Reactions

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SKU: RN7594 Category:
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