SeqVersa™ Quant Kit for Illumina® ensures accurate quantification of next-generation sequencing (NGS) libraries, enabling optimal loading and consistent sequencing performance on Illumina platforms.
$118.00 – $505.00Price range: $118.00 through $505.00
SeqVersa™ Quant Kit for Illumina® is designed to provide precise and reliable quantification of NGS libraries, ensuring optimal loading for consistent sequencing performance. Accurate library quantification is critical for achieving high-quality data and maximizing the efficiency of sequencing runs on Illumina® platforms.
This kit employs qPCR-based quantification using carefully calibrated standards and specific primer sets to measure the concentration of sequencing-ready libraries. Unlike other methods that may not accurately reflect the number of sequenceable molecules, the SeqVersa™ Quant Kit for Illumina® ensures that only the adapter-ligated library fragments are quantified, providing a true representation of library concentration. Key features of the SeqVersa™ Quant Kit for lllumina® include:
High Sensitivity and Accuracy: The qPCR-based approach offers high sensitivity, allowing for accurate quantification even at low library concentrations. This precision helps in avoiding under or overloading of the sequencer, which can compromise data quality.
Ease of Use: The kit includes all necessary components for library quantification, including calibrated DNA standards, qPCR primers, and a user-friendly protocol. This streamlined workflow ensures that even users with minimal experience in qPCR can achieve reliable results.
Consistency Across Runs: By providing consistent quantification, the SeqVersa™ Quant Kit for Illumina® minimizes variability between sequencing runs, enabling reproducible results and facilitating comparative analyses across experiments.
The SeqVersa™ Quant Kit for Illumina® is compatible with all Illumina sequencing platforms and can be seamlessly integrated into existing library preparation workflows. It is an essential tool for researchers looking to optimize their sequencing experiments and obtain high-quality, reliable data.
● Calibrated DNA Standards
● qPCR Primers
● qPCR Master Mix
● Quantification Method: qPCR-based
● Compatibility: All Illumina sequencing platforms
● Sensitivity: High sensitivity for low-concentration libraries
● Storage Conditions: -20°C for all reagents
● Accurate quantification of NGS libraries
● Optimization of sequencing library loading
● Consistent sequencing performance across runs
● High-quality data generation for various NGS applications
The kit is used for precise and reliable quantification of NGS libraries to ensure optimal loading and consistent performance on Illumina® sequencing platforms.
The kit employs a qPCR-based approach with calibrated DNA standards and specific primers to measure the concentration of sequencing-ready, adapter-ligated library fragments, providing a true representation of library concentration.
Yes, the kit is compatible with all Illumina® sequencing platforms, making it versatile for various sequencing applications and workflows.
The kit offers high sensitivity and accuracy, ease of use, and consistency across sequencing runs, ensuring high-quality data generation and optimal sequencing performance.
The kit includes calibrated DNA standards, qPCR primers, qPCR master mix, and a user manual to facilitate accurate and reliable library quantification.
All components should be stored at -20°C to maintain their stability and performance, as indicated in the user manual.
Reactions | 100, 500 |
---|