The SeqVersa™ PCR Free DNA Library Prep Kit provides a streamlined solution for preparing high-quality DNA libraries without the need for PCR amplification, ensuring minimal bias and high fidelity in sequencing results.
SeqVersa™ PCR Free DNA Library Prep Kit
Price range: $656.00 through $2,495.00
Purchase this item and get Product Description
The SeqVersa™ PCR Free DNA Library Prep Kit is designed to offer researchers a robust and efficient method for preparing DNA libraries without the need for PCR amplification. By eliminating the PCR step, this kit reduces the potential for bias and artifacts that can arise from amplification, thereby preserving the original representation of the genome. This makes it an ideal choice for applications requiring high fidelity and accurate sequencing, such as whole genome sequencing, metagenomics, and other genomic studies. This kit includes optimized reagents for end repair, A-tailing, adapter ligation, and sample purification, ensuring high-quality libraries with minimal hands-on time. The inclusion of sample purification beads allows for efficient cleanup and concentration of the prepared libraries, enhancing the overall workflow efficiency. The protocol is streamlined and user-friendly, making it accessible for researchers at all experience levels.
With the SeqVersa™ PCR Free DNA Library Prep Kit, researchers can achieve consistent and reliable results, ensuring their sequencing data accurately reflects the original DNA sample. This kit is compatible with a wide range of DNA inputs and is particularly suited for high-throughput sequencing applications.
Kit Contents
● End Repair Reaction Buffer
● End Repair Enzyme Mix
● A-Tailing Reaction Buffer
Technical Parameters
● Input DNA Quantity: 50 ng to 1 µg
● Library Yield: Sufficient for multiple sequencing runs
● Processing Time: Approximately 3 hours
● Storage Conditions: -20°C for all reagents
● Compatibility: Compatible with various DNA samples (e.g., genomic DNA, metagenomic DNA)
Applications
● Whole genome sequencing
● Metagenomics
● Genomic studies requiring high fidelity
● Any sequencing application where PCR bias needs to be minimized
Product FAQs
What is the advantage of using a PCR-free library prep kit?
PCR-free library prep reduces the potential for bias and artifacts introduced during amplification, preserving the original representation of the genome.
What DNA input range is compatible with the SeqVersa™ PCR Free DNA Library Prep Kit?
The kit is designed to work with DNA inputs ranging from 50 ng to 1 µg.
How long does it take to prepare a library using this kit?
The entire process takes approximately 3 hours.
Is this kit compatible with various types of DNA samples?
Yes, the kit is compatible with a wide range of DNA samples, including genomic DNA and metagenomic DNA.
| Reactions | 24, 96 |
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