Oligo-dT Primers are used for cDNA synthesis, specifically binding to the poly-A tail of mRNA, facilitating reverse transcription into cDNA.
Oligo-dT Primers
$38.10
Purchase this item and get Product Description
Oligo-dT Primers are essential tools in molecular biology for the synthesis of complementary DNA (cDNA) from messenger RNA (mRNA). These primers specifically anneal to the poly-A tail of mRNA molecules, providing a starting point for reverse transcription. This is crucial for converting mRNA into stable and amplifiable cDNA, which can be used in various downstream applications, including PCR, qPCR, and cloning. The primers are highly effective in capturing the mRNA population from total RNA, ensuring that the resulting cDNA is representative of the expressed genes. This makes them particularly valuable in gene expression studies, where accurate and efficient CDNA synthesis is critical. Each primer batch is rigorously tested for purity and performance, ensuring consistent results in every experiment. The high specificity and binding efficiency of these primers make them a reliable choice for researchers aiming to study gene expression patterns, mRNA abundance, and other molecular biology investigations.
Kit Contents
● 100 µl of Oligo-dT Primers (0.4 µg/µl)
Technical Parameters
● Concentration: 0.4 µg/µl
● Length: 20 nucleotides
● Highly specific binding to the poly-A tail of mRNA
Applications
● cDNA synthesis from mRNA
● Gene expression analysis
● RT-PCR and qRT-PCR
● Cloning of cDNA
Product FAQs
What is the primary use of Oligo-dT Primers?
They are used for synthesizing cDNA from mRNA by binding to the poly-A tail.
What concentration are the Oligo-dT Primers supplied in?
They are provided at a concentration of 0.4 µg/µl.
Are these primers suitable for high-throughput applications?
Yes, they are compatible with high-throughput workflows.
What downstream applications can the cDNA synthesized with these primers be used for?
The cDNA can be used for PCR, qPCR, gene expression studies, and cloning.
| Quantity | 100 µl(0.4 µg/ul) |
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