The MethylMaster™ Methylated DNA Enrichment Kit selectively enriches methyl-CpG DNA from fragmented genomic DNA, utilizing the high-affinity binding properties of the methyl-CpG binding domain of human MBD2 protein fused to the Fc-tail of human IgG1.
MethylMaster™ Methylated DNA Enrichment Kit
$489.00
Purchase this item and get Product Description
The MethylMaster™ Methylated DNA Enrichment Kit is a specialized tool designed to facilitate the selective enrichment of double-stranded methyl-CpG DNA from fragmented genomic DNA samples. This kit employs the methyl-CpG binding domain (MBD) of human MBD2 protein, which is fused to the Fc-tail of human IgG1. This fusion protein offers high-affinity binding to methyl-CpG sites, allowing for the efficient and specific isolation of methylated DNA regions. The protocol provided with the kit is user-friendly and can be completed in less than two hours, making it an ideal choice for researchers who need rapid and reliable results.
The kit’s design ensures minimal non-specific binding and high recovery rates, making it suitable for downstream applications such as PCR, sequencing, and library preparation for high-throughput sequencing. By enabling the enrichment of methylated DNA, this kit supports studies on DNA methylation patterns, epigenetic regulation, and the identification of differentially methylated regions across various biological samples. With the ability to process up to 100 μg of fragmented input DNA per reaction, the MethylMaster™ Methylated DNA Enrichment Kit provides a robust solution for epigenetic research, offering high sensitivity and specificity in detecting methylated DNA.
Kit Contents
● MBD2-Fc Protein
● Protein A Magnetic Beads
● Binding Buffer
● Wash Buffer
● Elution Buffer
Technical Parameters
● Input DNA: Up to 100 μg fragmented genomic DNA
● Enrichment Target: Methyl-CpG sites in double-stranded DNA
● Binding Capacity: High-affinity binding via MBD2-Fc fusion protein
● Process Time: Less than 2 hours
● Application Compatibility: PCR, sequencing, library preparation
Applications
● DNA methylation studies
● Epigenetic research
● Differentially methylated region identification
● PCR
● Sequencing
● High-throughput sequencing library preparation
Product FAQs
What is the main application of the MethylMaster™ Methylated DNA Enrichment Kit?
The main application is the enrichment of methyl-CpG DNA from fragmented genomic DNA, facilitating studies on DNA methylation and epigenetic regulation.
How much input DNA can be used with this kit?
The kit can process up to 100 µg of fragmented genomic DNA per reaction.
What makes the MethylMaster™ Methylated DNA Enrichment Kit specific for methyl-CpG sites?
The kit uses the MBD2-Fc fusion protein, which has high-affinity binding properties specific to methyl-CpG sites in double-stranded DNA.
Can the enriched DNA be used for high-throughput sequencing?
Yes, the enriched DNA is suitable for various downstream applications, including high-throughput sequencing, PCR, and library preparation.
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