Fusion Taq Seq PCR Master Mix delivers exceptional specificity, sensitivity, and yield for high-fidelity PCR, making it ideal for next-generation sequencing and other demanding applications.
FusionTaq™ Seq PCR Master Mix
Price range: $103.00 through $410.00
Purchase this item and get Product Description
The FusionTaq™ Seq PCR Master Mix is a high-performance solution designed to meet the stringent demands of next-generation sequencing (NGS) and other advanced molecular biology applications. Engineered with a unique hot start mechanism, this master mix ensures maximum specificity by minimizing non-specific amplification and primer-dimer formation.
FusionTaq™ Seq PCR Master Mix provides unparalleled accuracy, reducing the risk of errors in amplified DNA sequences. This polymerase features an enhanced buffer system that supports robust performance across a wide range of templates, including complex and GC-rich sequences. The mix is optimized for high sensitivity, allowing for the detection and amplification of low-abundance targets, which is critical for sequencing applications and rare variant detection.
In addition to its superior performance, FusionTaq™ Seq PCR Master Mix is exceptionally user-friendly, combining convenience with reliability. The all-in-one formulation includes dNTPs, MgCl2, and a high-fidelity polymerase, simplifying the setup and reducing the potential for contamination. The mix’s robust formulation also allows for a wide range of PCR conditions, providing flexibility in protocol design.
Whether you are preparing libraries for NGS, amplifying challenging templates, or conducting routine PCR, FusionTaq™ Seq PCR Master Mix ensures high yield, precision, and reproducibility, making it an indispensable tool in any molecular biology laboratory.
Key features include:
Hot Start Mechanism. Ensures maximum specificity by preventing non-specific amplification.
Enhanced Buffer System: Optimized for robust performance with complex and GC-rich templates.
User-Friendly: All-in-one formulation simplifies setup and reduces contamination risk.
Versatile: Suitable for a wide range of PCR applications, including NGS library preparation and rare variant detection.
Kit Contents
● FusionTaq™ Seq PCR Master Mix (2X)
● Positive Control Template
Technical Parameters
● Concentration: 2X master mix
● dNTPs: Included
● MgCl2: Included
● Storage Conditions: -20°C for all components
● Template Compatibility: Effective with a wide range of DNA templates,
including complex and GC-rich sequence
Applications
● Next-Generation Sequencing (NGS) library preparation
● High-fidelity PCR
● Amplification of complex and GC-rich templates
● Rare variant detection
● Routine PCR
Product FAQs
What is FusionTaq™ Seq PCR Master Mix used for?
It is used for high-fidelity PCR applications, including NGS library preparation, amplification of complex templates, and rare variant detection.
How does the hot start mechanism work in FusionTaq™ Seq PCR Master Mix?
The hot start mechanism prevents non-specific by keeping the polymerase inactive at lower temperatures, which reduces primer-dimer formation and non-specific binding.
What are the storage requirements for the FusionTaq™ Seq PCR Master Mix?
The master mix and all components should be stored at -20°C to maintain their stability and performance.
Can FusionTaq™ Seq PCR Master Mix handle GC-rich templates?
Yes, the enhanced buffer system in the master mix is optimized to handle complex and GC-rich templates effectively.
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