Automated solution for high-throughput purification of viral RNA and DNA.
The VersaKit™ Viral Extraction for AutEx™ offers a streamlined and automated solution for the purification of viral RNA and DNA from various sample types, including blood, tissues, swabs, and body fluids. By harnessing the power of silica-membrane technology in a convenient 96-well format, this kit ensures efficient removal of contaminants and inhibitors, resulting in high-quality nucleic acids ready for downstream analysis.
Utilizing the AutEx™ system, the kit enables simple and reliable processing, leading to significant cost and time savings. With its ability to process 24-96 samples in increments of 8, users can achieve consistent and high yields of purified viral nucleic acids without compromising on quality or reliability. The purified nucleic acids are suitable for analysis by real-time PCR or RT-PCR, making it an ideal choice for a wide range of molecular biology applications.
The principle behind the VersaKit™ Viral Extraction for AutEx™ lies in its combination of silica-based membrane binding properties and high-throughput automation. Samples undergo lysis under denaturing conditions, facilitated by AvamⓇ Proteinase K and specialized buffers, ensuring nuclease inactivation. The lysate is then transferred to a 96 well plate where nucleic acids are selectively adsorbed onto silica membranes while contaminants are removed through efficient washing steps. Finally, the purified nucleic acids are eluted, ready for downstream applications.
With specifications catering to varying sample volumes and processing durations, the kit offers flexibility and efficiency. The eluted nucleic acids are of high quality and yield, as demonstrated by reliable performance in PCR and RT-PCR analyses across a range of sample types.
Overall, the VersaKit™ Viral Extraction for AutEx™ provides a robust and user-friendly solution for automated viral nucleic acid purification, offering researchers and clinicians the confidence and convenience needed for their molecular biology workflows.
● 96 well plates
● Avam® Proteinase K
● Carrier RNA
● Buffers
● Number of samples: 24–96 samples (to be processed in increments of 8)
● Sample input volume: Up to 200 μl for blood and other body fluids; up to 20 mg for tissues
● Elution volume: 100 μl
● Duration: 96 samples in approximately 145 minutes; 24 samples in approximately 90 minutes
● PCR
● Real-time PCR
● RT-PCR
● Next-generation sequencing
Yes, the VersaKit™ Viral Extraction for AutEx™ is suitable for processing clinical samples, including blood, tissues, swabs, and body fluids.
Absolutely, the purified nucleic acids are ready for downstream PCR, real-time PCR, RT-PCR, and other molecular biology applications.
The processing time for 96 samples is approximately 145 minutes, providing a fast and efficient workflow for high-throughput applications.
Yes, the kit is designed for use with the AutEx™ system, which automates the purification process, ensuring consistent and reliable results.
Quantity | 480 preps |
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