Extract DNA and RNA from stool or gut samples with improved Inhibitor Cleanup Technology for metagenomic and metatranscriptomic analysis.
The VersaKit™ Plus Fecal offers a comprehensive solution for the simultaneous extraction of DNA and RNA from stool or gut samples. Utilizing a proprietary protocol with Inhibitor Cleanup Technology, this kit efficiently separates RNA and DNA into different eluates, making it ideal for metagenomic and metatranscriptomic analyses.
Featuring optimized lysis chemistry and bead-beating technology, the kit ensures high yields of high-quality DNA and RNA from challenging samples containing inhibitory substances such as polysaccharides, heme compounds, and bile salts. The extracted nucleic acids are suitable for downstream applications including PCR, RT-PCR, qPCR, and next-generation sequencing (NGS) such as 16S rRNA sequencing, metagenome, and metatranscriptome analysis.
The VersaKit™ Plus Fecal allows for the simultaneous purification of microbial DNA and RNA from stool samples, ensuring comprehensive microbial analysis without the need for multiple purification steps. The kit is a complete solution from sample collection to extraction. Stool samples can be stabilized at room temperature, preserving the integrity of nucleic acids until processing.
● 50 PulseBead™ Tubes
● 50 Magnetic Bead Spin Columns
● 50 Magnetic Bead RNA Spin Columns
● 150 Collection Tubes (2 mL)
● 100 Elution Tubes (1.5 mL)
● 100 Microcentrifuge Tubes (2 mL)
● Nuclease-free Water, Reagents, and Buffers
● Sample types: Stool, gut material
● Format: 2 mL tubes
● Sample size: 50-200 mg
● Processing: PulseBead™ Tubes (mix of zirconia beads)
● Storage temperature: Store CD2 at 2-8°C. All other kit reagents and components should be stored at room temperature (15-25°C).
● PCR
● RT-PCR
● qPCR
● Next-generation sequencing (NGS)
● Metagenomic and metatranscriptomic analysis
Yes, the VersaKit™ Plus Fecal enables simultaneous extraction of DNA and RNA from stool or gut samples.
The ICT effectively removes inhibitory substances commonly found in stool samples, ensuring efficient nucleic acid extraction.
Yes, the extracted DNA and RNA are suitable for a range of downstream applications including metagenomic and metatranscriptomic studies.
Quantity | 50 preps |
---|