VersaKit™ NA & Protein All-Rounder

VersaKit™ NA & Protein All-Rounder

$922.00

Simultaneously isolate DNA, RNA, and protein from cells or tissues with maximal recovery and minimal workflow steps.

Purchase this item and get 92 Points
Purchase this item and get 92 Points
Product Description

The VersaKit™ NA & Protein All-Rounder revolutionizes sample preparation by offering simultaneous extraction of DNA, RNA, and protein from the same cellular or tissue source. Developed for molecular biology applications, this kit ensures maximal recovery of each analyte from precious samples, saving time through a streamlined workflow while maintaining comparable data quality as all analytes originate from the same source.

Utilizing innovative technology, the VersaKit™ NA & Protein All-Rounder eliminates the need for organic solvents like phenol and acetone, ensuring user safety and environmental friendliness. The kit’s performance is exemplified by its ability to deliver optimal results in real-time PCR and RT-PCR analyses, as demonstrated by comparable CT values to tissues stabilized in liquid nitrogen. Moreover, the total protein purified using the kit is suitable for SDS-PAGE and western blotting, ensuring versatility in downstream applications.

The VersaKit™ NA & Protein All-Rounder caters to various downstream applications, including PCR, RT-PCR, real-time PCR, real-time RT-PCR, Southern-, Northern-, and Western Blotting, Microarrays, Genotyping, as well as 1D and 2D gel electrophoresis. With an elution volume ranging from 30 to 50µl for RNA and 100 to 200µl for DNA, this kit offers flexibility to suit diverse experimental needs.

In summary, the VersaKit™ NA & Protein All-Rounder empowers researchers with a comprehensive solution for sample preparation, ensuring maximal recovery of DNA, RNA, and protein from challenging samples, and delivering reliable results across a spectrum of molecular biology applications.

Kit Contents

● VersaKit™ DNA Spin Columns
● NuAction™ Spin Columns
● Collection Tubes
● RNase-Free Reagents and Buffers

Technical Parameters​

● Applications: PCR, RT-PCR, real-time PCR, real-time RT-PCR, Southern-, Northern-, Western Blotting, Microarrays, Genotyping, 1D
and 2D gel electrophoresis
● Main sample type: Easy-to-lyse tissue or cells
● Elution volume: RNA: 30 – 50 µl, DNA: 100 – 200 µl
● Purification of analytes: Total RNA, genomic DNA, and western-grade proteins
● Format: Spin column
● Yield: Varies, depends on sample type
● Time per run or per prep: 60 min
● Processing: Manual (centrifugation)
● Technology: Silica technology, Protein precipitation
● Sample amount: Up to 30 mg tissue or 1 x 10e7 cells

Applications

● PCR
● RT-PCR
● Real-time PCR
● Real-time RT-PCR
● Southern Blotting
● Northern Blotting
● Western Blotting
● Microarrays
● Genotyping
● 1D and 2D gel electrophoresis

Product FAQs
Can I use this kit for both cultured cells and tissue samples?

Yes, the Versakit™ NA & Protein All-Rounder is compatible with both cultured cells and tissue samples, ensuring versatility in sample types.

How long does it take to process samples with this kit?

The processing time per run or prep is approximately 60 minutes, making it efficient for high-throughput experiments.

Is it necessary to use organic solvents like phenol for purification?

No, this kit does not require the use of organic solvents such as phenol, ensuring user safety and environmental friendliness.

What downstream applications are supported by the purified DNA, RNA, and protein?

The purified analytes are suitable for a wide range of applications including PCR, RT-PCR, real-time PCR, real-time RT-PCR, Southern-, Northern-, and Western Blotting, Microarrays, Genotyping, and gel electrophoresis.

Quantity

50 preps
SKU: NA3408 Category:
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