VersaKit™ FFPE DNA/RNA

$950.00

VersaKit™ FFPE DNA/RNA enables simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections, ensuring maximum output with minimal sample consumption for comprehensive molecular biology analysis.

Purchase this item and get 95 Points
Purchase this item and get 95 Points
Product Description

The VersaKit™ FFPE DNA/RNA stands as a cornerstone in molecular biology research, offering a comprehensive solution for the simultaneous purification of genomic DNA and total RNA from FFPE tissue sections. Unlike traditional methods that often require separate workflows for DNA and RNA extraction, this innovative kit streamlines the process by allowing purification of both nucleic acid types from the same sample. This unified approach not only saves time and resources but also ensures that genomic and transcriptomic data obtained from FFPE samples are directly comparable, fostering more robust analyses and interpretations.

One of the key strengths of the VersaKit™ FFPE DNA/RNA lies in its ability to preserve the integrity of nucleic acids during the purification process. FFPE samples, notorious for their heavily fragmented nucleic acids and chemical modifications resulting from formaldehyde fixation, present unique challenges in molecular biology research. However, the kit’s patent-pending solubilization method effectively addresses these challenges, enabling the release of DNA and RNA while minimizing degradation and maintaining sample integrity. This feature is particularly crucial for downstream applications that demand high-quality nucleic acids, such as real-time PCR and Pyrosequencing.

Moreover, the VersaKit™ FFPE DNA/RNA offers researchers unparalleled flexibility in experimental design and execution. By providing purified DNA and RNA from the same FFPE sample, it facilitates a wide range of applications, including PCR, qPCR, real-time RT-PCR, microarray analysis, and next-generation sequencing (NGS). Whether investigating gene expression profiles, identifying genetic mutations, or conducting epigenetic studies, researchers can rely on the kit to deliver consistent and reliable results across various molecular biology assays.

Furthermore, the kit’s compatibility with automation platforms such as the Avam® AutEx™ Platform enhances workflow efficiency and reproducibility, making it an ideal choice for laboratories with high sample throughput requirements. The streamlined purification process, coupled with automated operation, minimizes hands-on time and reduces the risk of human error, thereby optimizing resource utilization and accelerating research progress. In summary, the VersaKit™ FFPE DNA/RNA represents a cutting-edge solution for the simultaneous extraction of DNA and RNA from FFPE samples, empowering researchers to unlock valuable insights into the molecular mechanisms underlying disease pathogenesis, biomarker discovery, and personalized medicine. With its superior performance, versatility, and user-friendly workflow, the kit exemplifies excellence in molecular biology research and sets a new standard for nucleic acid purification from FFPE tissues.

Kit Contents

● 50 NuAction™ Elution Spin Columns
● 50 VersaKit™ Elution Spin Columns
● Collection Tubes
● RNase-Free Reagents and Buffers

Technical Parameters​

● Applications: PCR, qPCR, real-time RT-PCR, microarray
● Elution Volume: RNA (14-30 µl), DNA (30-100 µl)
● Purification Targets: Total RNA, miRNA, poly A+ mRNA, DNA, or protein
● Processing: Manual (centrifugation)
● Format: Spin column
● Number of Preps per Run: 50
● Sample Amount: Max. 410 µm sections or 220 µm sections
● Technology: Silica technology
● Time per Run or per Prep: 6 hours for 10 samples, including sectioning
● Yield: Varies
● Main Sample Type: FFPE tissue samples

Applications

● PCR
● qPCR
● Real-time RT-PCR
● Microarray analysis
● Comparative genomic hybridization (CGH)
● Next-generation sequencing (NGS)

Product FAQs
Can this kit effectively separate RNA and DNA from FFPE samples?

Yes, the VersaKit™ FFPE DNA/RNA employs a patented solubilization method to efficiently release and purify both RNA and DNA from the same FFPE sample, ensuring effective separation.

What downstream applications are compatible with the purified nucleic acids?

The purified DNA and RNA can be used in a variety of downstream applications, including PCR, qPCR, real-time RT-PCR, microarray analysis, and NGS.

Is the kit suitable for processing FFPE samples of varying ages?

Yes, the VersaKit™ FFPE DNA/RNA is optimized to reverse formaldehyde modification without further degradation of DNA and RNA, making it suitable for FFPE samples of different ages.

How many samples can be processed in a single run with this kit?

The kit provides materials for processing 50 samples in a single run, making it suitable for high-throughput applications in molecular biology research.

Quantity

50 preps

SKU: PE6032 Category:
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