The VersaKit™ RNA Blood Kit offers rapid purification of high-quality RNA from up to 1.5 mL of fresh human blood, without the need for organic extraction or alcohol precipitation.
VersaKit™ RNA Blood Kit
$496.00
Purchase this item and get Product Description
The VersaKit™ RNA Blood Kit is a powerful tool for molecular biology applications, providing a streamlined method for the purification of cellular RNA. With its silica-membrane-based technology, the kit offers a rapid and efficient solution for isolating RNA from various anticoagulant-stabilized blood samples, including those treated with citrate, heparin, or EDTA.
Utilizing spin-column format, the VersaKit™ RNA Blood Kit simplifies the RNA purification process, eliminating the need for labor-intensive organic extraction or alcohol precipitation steps. This results in consistent and high yields of RNA, free from contaminants and enzyme inhibitors.
The kit’s performance is characterized by its ability to completely remove RNases and other contaminants, ensuring the isolation of high-quality RNA suitable for a wide range of downstream applications. Whether it’s RT-PCR, real-time RT-PCR, cDNA synthesis, or blotting procedures, the purified RNA is ready for immediate use without any detectable PCR inhibition.
The principle underlying the VersaKit™ RNA Blood Kit lies in its silica-membrane technology, which selectively binds RNA while allowing contaminants to pass through. This method ensures the removal of PCR inhibitors, such as divalent cations and proteins, through efficient wash steps, resulting in pure RNA eluted in water or a provided buffer.
Moreover, the VersaKit™ RNA Blood Kit offers versatility beyond blood samples. It can also be used to purify RNA from tissues and cultured cells, as well as to separate RNA from proteins, salts, and other reaction components post-enzymatic reactions.
With its user-friendly procedure and fast processing time of less than one hour, the VersaKit™ RNA Blood Kit is a valuable asset for researchers seeking reliable and high-quality RNA extraction for their molecular biology studies.
Kit Contents
● 50 VersaKit™ Mini Spin Columns
● 50 Disruption Spin Columns
● Collection Tubes (1.5 mL and 2 mL)
● RNase-free reagents and buffers
Technical Parameters
● Applications: PCR, real-time PCR, microarray
● Elution volume: 30–100 µl
● Main sample type: Whole blood, tissue
● Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein: Cellular RNA
● Sample amount: 50–1.5 mL
● Format: Spin columns
● Processing: Manual (centrifugation)
● Time per run or per prep: Less than 1 hour
● Technology: Silica technology
● Yield: 1–5 µg
Applications
● RT-PCR
● Real-time RT-PCR
● cDNA synthesis
● Northern, dot and slot blotting
● RNase/S1 nuclease protection
● Differential display
● Poly A+ RNA selection
● Primer extension
● Purification of total RNA from tissues and cultured cells
● Separation of RNA from proteins, salts, and other reaction components after enzymatic reactions
Product FAQs
What is the maximum sample volume for RNA extraction using the VersaKit™ RNA Blood Kit?
The kit is optimized for the purification of RNA from up to 1.5 mL of fresh, whole human blood.
Can the purified RNA be used directly in downstream applications?
Yes, the RNA purified using the VersaKit™ RNA Blood Kit is ready to use in various downstream applications without the need for additional purification steps.
How long does the RNA purification procedure take?
The RNA purification procedure can be completed in less than one hour, providing a rapid method for obtaining high-quality RNA from blood samples.
Are there any specific storage conditions for the RNA obtained with this kit?
The purified RNA should be stored at -70°C or below for long-term storage to maintain its stability and integrity for future experiments.
| Quantity | 50 preps |
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