ParaPrep™ Tissue Kit

$674.00

The ParaPrep™ Tissue Kit offers efficient, automated purification of high-quality total RNA from tissue samples. Ideal for consistent and reliable RNA yields in translational and discovery research.

Purchase this item and get 67 Points
Purchase this item and get 67 Points
Product Description

The ParaPrep™ Tissue Kit offers a seamless solution for automated purification of total RNA from standard tissue samples, providing researchers with a reliable tool for molecular biology applications. This kit ensures consistent yields of high-quality RNA, facilitating reproducible results.

This comprehensive kit contains all necessary reagents and labware for efficient RNA purification from up to 10 mg of tissue samples, employing advanced magnetic particle technology. Prefilled and sealed reagent cartridges expedite the loading process, enhancing workflow efficiency and minimizing contamination risks.

Consistently delivering high yields of pure RNA, the ParaPrep™ Tissue Kit ensures reliable performance across various tissue types. Demonstrated by figures depicting efficient purification and reproducible yields, the kit consistently provides RNA of exceptional quality, ideal for diverse downstream applications.

Utilizing silica-coated magnetic particles, the ParaPrep™ Tissue Kit operates seamlessly with automated protocols, ensuring optimal purification conditions and minimal user intervention. The automated procedure guarantees high repeatability and linear yields, accommodating varying sample weights and tissue types.

The workflow begins with tissue lysis and homogenization in Buffer RLT, followed by automated purification using the EZ1&2 RNA procedure. Integrated DNase digestion eliminates genomic DNA contamination, enhancing RNA purity. Total RNA is eluted in customizable volumes, ready for immediate use in downstream applications.

The high-quality RNA obtained with the ParaPrep™ Tissue Kit is suitable for a diverse range of applications, including gene expression analysis using real-time RT-PCR and microarray technologies, as well as oncology and pathology research. Researchers can rely on the integrity and consistency of the purified RNA for accurate molecular analyses.

Kit Contents

● Reagent cartridges (Tissue RNA)
● Disposable filter-tips
● Disposable tip-holders
● Sample tubes (2 mL)
● Elution tubes (1.5 mL)
● Tissue Lysis Buffer
● Avam® RNase-free DNase I
● Nuclease-free water

Technical Parameters​

● Applications: RT-PCR, digital PCR (dPCR), next-generation sequencing (NGS)
● Elution Volume: 50 or 100 µl
● Main Sample Type: Tissue
● Processing: Automated with an EZ1 Advanced XL instrument
● Analyte: Total RNA, including miRNA and mRNA
● Sample Amount: Up to 30 mg (frozen) or 15 mg (stabilized) tissue or 10 mg frozen spleen or lung tissue, or up to 5 x 10^6 cells
● Technology: Magnetic-particle technology

Applications

● Gene expression analysis using real-time RT-PCR technologies
● Gene expression analysis using microarray technologies
● Oncology and pathology research

Product FAQs
What distinguishes the ParaPrep™ Tissue Kit from other purification kits?

The ParaPrep™ Tissue Kit offers automated purification of high-quality RNA from tissue samples, ensuring reliable performance and consistent results without the need for manual intervention.

Can the ParaPrep™ Tissue Kit accommodate different tissue types?

Yes, the kit is designed to purify RNA from various tissue types, ensuring reliable performance and consistent yields across different sample matrices.

How does the kit ensure RNA purity during the automated purification process?

The kit features presealed reagent cartridges and automated protocols, minimizing contamination risks and ensuring the integrity of RNA samples for downstream applications.

What downstream applications can be performed with RNA purified using this kit?

The high-quality RNA obtained with the ParaPrep™ Tissue Kit is suitable for gene expression analysis, oncology research, pathology studies, and other molecular biology applications requiring pure RNA samples.

Quantity

48 preps

SKU: PE6821 Category:
Updating
  • No products in the cart.