Simplify RNA and miRNA purification from cells and tissues with efficient enzymatic removal of genomic DNA and no use of toxic phenol. Process up to 24 samples simultaneously for enhanced workflow efficiency.
ParaPrep™ miRNA Tissue/Cells Kit
$535.00
Purchase this item and get Product Description
The ParaPrep™ miRNA Tissue/Cells Kit revolutionizes the extraction of total RNA, including miRNA and small RNA, from an array of human or animal tissues and cultured cells. It offers a comprehensive solution that combines efficiency, safety, and convenience, ensuring researchers achieve high-quality RNA yields consistently. Unlike traditional methods, this kit eliminates the need for toxic phenol, enhancing laboratory safety while maintaining the integrity of RNA molecules.
One of the standout features of the ParaPrep™ miRNA Tissue/Cells Kit is its advanced enzymatic removal of genomic DNA, ensuring unparalleled purity and reliability in downstream applications. By efficiently removing genomic DNA contaminants, researchers can confidently perform sensitive molecular analyses without interference, guaranteeing accurate and reproducible results.
Designed to streamline laboratory workflows, this kit allows for the simultaneous processing of up to 24 samples, facilitating high-throughput applications and enabling easy intra-run comparability. Whether working with challenging tissue types such as fibrous or fatty tissues, the ParaPrep™ miRNA Tissue/Cells Kit consistently delivers exceptional performance, yielding comparable results to manual column-based protocols.
The workflow begins with manual lysis in Buffer RLT, preserving RNA molecules and ensuring their protection during subsequent processing steps. The addition of Proteinase K facilitates complete tissue lysis, even with hard-to-lyse samples, ensuring maximum RNA recovery.
During the purification process, RNA is treated with DNase to eliminate any residual genomic DNA, further enhancing the purity of the extracted RNA. Subsequent wash steps remove contaminants that could potentially interfere with downstream enzymatic reactions. Once the purification process is complete, researchers obtain highly pure RNA or miRNA, ready for a wide range of downstream applications.
The versatility of RNA and miRNA purified using the ParaPrep™ miRNA Tissue/Cells Kit makes it an invaluable tool for various molecular biology applications, including RT-PCR, digital PCR (dPCR), and next-generation sequencing (NGS). With its robust performance, ease of use, and exceptional results, this kit empowers researchers to advance their molecular biology research with confidence and efficiency.
Kit Contents
● ParaPrep™ RNA/miRNA Tissue/Cells cartridge
● Filter tips and holders
● Tubes
● Avam® RNase-free DNase
● Cell Lysis Buffer
● Avam® Proteinase K
Technical Parameters
● Purifies RNA and miRNA from human and animal cells and tissues
● Does not use toxic phenol
● Efficient enzymatic removal of protein and genomic DNA
● Processes up to 24 samples simultaneously for easy intra-run comparability
● Prevents RNase contamination with prefilled cartridges and closed workdeck
● Applications: RT-PCR, dPCR, NGS
● Elution Volume: 50 or 100 µl
● Main Sample Type: Cells, tissue
● Analyte: Total RNA, including miRNA and mRNA
● Sample Amount: Up to 30 mg (frozen) or 15 mg (stabilized) tissue, or 10 mg frozen spleen or lung tissue, or up to 5 x 10^6 cells
● Technology: Magnetic-particle technology
Applications
● Highly suitable for RT-PCR, digital PCR (dPCR), or next-generation
sequencing (NGS)
Product FAQs
Can the ParaPrep™ miRNA Tissue/Cells Kit process difficult tissue types?
Yes, the kit can isolate total RNA, including small RNA, from challenging tissue types such as fibrous or fatty tissues with comparable efficiency.
How does the kit ensure genomic DNA removal during the purification process?
The protocol includes efficient enzymatic removal of genomic DNA, ensuring minimal contamination and yielding high-quality RNA suitable for downstream applications.
Can this kit handle multiple samples at once?
Yes, the kit can process up to 24 samples simultaneously, facilitating easy intra-run comparability and enhancing workflow efficiency.
What downstream applications are compatible with RNA and miRNA purified using this kit?
RNA and miRNA purified with the ParaPrep™ miRNA Tissue/Cells Kit are highly suitable for RT-PCR, digital PCR (dPCR), and next-generation sequencing (NGS).
| Quantity | 48 preps |
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