NuAction™ Ultra Microbiome kit

$0.00

Streamline the isolation of high-quality microbial RNA from challenging microbiome samples with the NuAction™ Ultra Microbiome Kit, featuring advanced Inhibitor Cleanup Technology for efficient purification.

Product Description

The NuAction™ Ultra Microbiome kit is an essential tool for researchers working with challenging microbiome samples, where traditional RNA isolation methods often fail due to the presence of PCR inhibitors. This advanced kit offers a streamlined protocol that efficiently removes inhibitors while delivering high-quality RNA in less than 45 minutes.

Designed to handle a variety of sample types, including stool, dried feces, gut material, and contaminated buccal swabs and secretions, the NuAction™ Ultra Microbiome kit empowers researchers to explore the complex microbial communities present in these samples with confidence. By effectively lysing host and microbial cells through bead beating technology and specialized lysis chemistry, this kit ensures thorough sample disruption and RNA release. A key feature of the NuAction™ Ultra Microbiome kit is its Inhibitor Removal Technology, which tackles common PCR inhibitors found in microbiome samples, such as digested food particles and heme from lysed red blood cells in stool. By efficiently eliminating these inhibitors, the kit produces RNA of exceptional purity, free from interference that could compromise downstream applications.

Moreover, the kit offers the flexibility to co-isolate miRNA and optionally extract DNA using a simple, on-column procedure. This feature not only streamlines the workflow but also enhances the kit’s versatility, allowing researchers to extract multiple nucleic acid types from the same sample simultaneously.

The robust performance of the NuAction™ Ultra Microbiome kit is backed by extensive validation studies, demonstrating its ability to consistently yield high-quality RNA from a range of microbiome samples. Figures showcasing electropherograms and RNA quality metrics underscore the reliability and reproducibility of the kit’s results, providing researchers with confidence in their experimental outcomes.

Additionally, the NuAction™ Ultra Microbiome kit is user-friendly and compatible with automation on the Avam® AutEx™ Platform, enabling seamless integration into high-throughput workflows. Whether conducting basic research, biomarker discovery, or clinical studies, researchers can rely on this kit to deliver reliable RNA results from even the most challenging microbiome samples.

In summary, the NuAction™ Ultra Microbiome kit offers a comprehensive solution for RNA isolation from complex microbiome samples, combining speed, efficiency, and superior performance to meet the diverse needs of microbiome researchers.

Kit Contents

● Silica Spin Filter Tubes
● Avam® Lyophilized DNase I
● Buffers and reagents for RNA isolation

Technical Parameters​

● Sample Types: Stool, gut material, dried feces, contaminated buccal swabs, and secretions
● Format: Silica Spin Filter Tubes
● Time per Run or per Prep: 45 minutes
● Sample Size: 0.25 g
● Throughput: 1-24 samples
● Bead Size: 0.1 mm glass
● Storage Temperature: Store Avam lyophilized DNase I at 4°C; all other kit reagents and components should be stored at room temperature (15-30°C)

Applications

● RT-PCR
● qPCR
● RNA-seq
● Microbiome research
● Biomarker discovery

Product FAQs
Can I use this kit for RNA isolation from other sample types?

Yes, while optimized for microbiome samples, this kit can also be used for RNA isolation from various other sample types.

Does the kit include Avam® DNase I for DNA removal?

Yes, the kit includes lyophilized Avam® DNase I for on-column DNA digestion during RNA purification.

Is the isolated RNA suitable for downstream applications like RNA-seq?

Yes, the RNA obtained using this kit is of high quality and suitable for a wide range of downstream applications, including RNA-seq.

Can I automate the RNA isolation process with this kit?

Yes, the NuAction™ Ultra Microbiome kit is compatible with automation on the Avam® AutEx™ Platform, streamlining your workflow and increasing efficiency.

Quantity

Inquire for the pricing of individual buffer components

SKU: NU1829 Category:
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