Enables the immediate stabilization and purification of high-quality RNA from tissue, cells, saliva, and bacteria, ensuring reliable gene expression data without degradation.
NuAction™ Stabilizer Kit
$1,865.00
Purchase this item and get Product Description
The NuAction™ Stabilizer Kit is designed to address the critical need for the immediate stabilization and subsequent isolation of high-quality RNA from a diverse range of sample types, including tissue, cells, saliva, and bacteria. Its innovative design incorporates NuAction™ Tissue Reagent, which acts swiftly to stabilize RNA upon sample collection, preserving the integrity of gene expression profiles and preventing alterations induced by sample manipulation.
This kit offers researchers a streamlined workflow for RNA stabilization and purification, eliminating the need for labor-intensive methods involving liquid nitrogen, dry ice, or phenol. By providing ready-to-use, high-quality total RNA within minutes, the NuAction™ Stabilizer Kit ensures reliable gene expression data for a wide array of downstream applications.
With the inclusion of NuAction™ Spin Columns utilizing silica-membrane technology, the kit enables the purification of up to 100 µg of RNA, ensuring ample yield for subsequent analyses. The versatility of this kit allows researchers to work with various sample types and preparations, accommodating different experimental requirements and providing consistent and reproducible results.
The NuAction™ Stabilizer Kit is not only highly efficient but also user-friendly, featuring a manual centrifugation process that is easy to follow. Additionally, the kit can be automated on the Avam® AutEx™ Platform, offering enhanced convenience and processing flexibility, particularly for high-throughput applications.
Whether researchers are investigating gene expression profiles in tissues, analyzing RNA from cultured cells, studying saliva samples for biomarkers, or examining bacterial gene expression, the NuAction™ Stabilizer Kit provides a comprehensive solution for RNA stabilization and purification. Its robust performance, coupled with compatibility with a wide range of downstream applications, makes it an indispensable tool for molecular biology research.
Kit Contents
● NuAction™ Stabilize Tissue Reagent (50 mL)
● NuAction™ Spin Columns
● Collection Tubes (1.5 mL and 2 mL)
● RNase-Free Reagents and Buffers
Technical Parameters
● Sample Types: Tissue, Bacteria, Cell, Saliva
● Sample Preparations: 50 (for tissue), 250 (for bacteria)
● RNA Yield: Varies depending on sample type and input amount
● Elution Volume: 30–100 µl
● Processing: Manual (centrifugation)
● Technology: Silica technology
Applications
● RNA-seq
● Quantitative, real-time RT-PCR
● End-point RT-PCR
● Northern, dot, and slot blotting
● Array analysis
● Poly A+ RNA selection
Product FAQs
What makes the NuAction™ Stabilizer Kit suitable for various sample types?
The kit includes NuAction™ Tissue Reagent, which enables the immediate stabilization of RNA in tissue samples, cells, saliva, and bacteria. This ensures reliable gene expression data without degradation, making it versatile for different experimental setups.
Can I store samples stabilized with stabilizer reagents under different conditions?
Yes, stabilized samples can be stored or transported under various conditions depending on the sample type. For example, cells in stabilizer reagents can be stored at different temperatures for up to 4 weeks, allowing flexibility in experimental workflows.
How does the kit prevent the degradation of mRNA during sample handling and processing?
The NuAction™ Tissue Reagent immediately stabilizes RNA upon sample collection, preventing changes in mRNA levels triggered by sample manipulation. This ensures the preservation of gene expression profiles and reliable downstream analysis.
What is the recommended storage condition for RNA purified using this kit?
RNA purified with the NuAction™ Stabilizer Kit can be stored long-term at -20°C or -80°C, ensuring the stability and integrity of the RNA for future experiments.
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