NuAction™ Fibrous Tissue Kit

NuAction™ Fibrous Tissue Kit

$553.00

Isolate high-quality RNA from fiber-rich tissue samples with the NuAction™ Fibrous Tissue Kit, featuring optimized protocols and efficient RNA extraction.

Purchase this item and get 55 Points
Purchase this item and get 55 Points
Product Description

The NuAction™ Fibrous Tissue Kit is a comprehensive solution for extracting total RNA from fibrous tissue samples, providing researchers with high yields of RNA suitable for a wide range of downstream applications. With its specialized protocols and innovative design, this kit ensures efficient RNA isolation from fiber-rich tissues, such as skeletal muscle, the heart, and the aorta.

The kit includes proteinase K for removing abundant protein in fiber-rich tissue samples, NuAction™ Spin Columns for purifying up to 100 µg of high-quality RNA, and a range of RNase-free reagents and buffers. The kit can be conveniently automated on the Avam AutEx™ platform, streamlining the RNA purification process and enhancing reproducibility.

The performance of the NuAction™ Fibrous Tissue Kit is unparalleled, with optimized protocols guaranteeing high yields of total RNA suitable for all downstream applications, including PCR, real-time PCR, and microarray analysis. Comparative data demonstrate superior RNA yields compared to other silica-based methods, making it the preferred choice for researchers working with fiber-rich tissue samples.

The principle behind the NuAction™ Fibrous Tissue Kit lies in its innovative combination of guanidine isothiocyanate lysis and silica-membrane purification technologies. This unique approach enables efficient RNA extraction from challenging fibrous tissues, breaking down proteins and genomic DNA to yield high-quality total RNA.

The procedure for using the NuAction™ Fibrous Tissue Kit is straightforward and can be completed in just 55 minutes. Fibrous tissue samples are lysed in a guanidine isothiocyanate buffer, treated with proteinase K, and then subjected to centrifugation to remove debris. RNA is then bound to the
NuAction™ Silica Membrane, followed by DNase treatment to remove traces of DNA. The purified RNA is eluted in 30-100 µl of RNase-free water, ready for downstream applications.

Kit Contents

● NuAction™ Mini Spin Columns
● Collection Tubes (1.5 mL and 2 mL)
● Avam® Proteinase K
● Avam® RNase-Free DNase I
● RNase-free Reagents and Buffers

Technical Parameters​

● Applications: PCR, real-time PCR, microarray
● Elution Volume: 30–100 µl
● Format: Spin Column
● Time per Run or per Prep: 55 minutes
● Purification of: Total RNA, miRNA, poly A+ mRNA, DNA or protein
● Processing: Manual
● Sample Amount: 0.5–30 mg
● Technology: Silica technology
● Main Sample Type: Fibre-rich tissue samples
● Yield: 5–12 µg

Applications

● PCR
● Real-time PCR
● Microarray

Product FAQs
Can I use the NuAction™ Fibrous Tissue Kit with fiber-rich tissue samples other than skeletal muscle, heart, and the aorta?

Yes, the kit is optimized for use with a variety of fiber-rich tissues, making it suitable for a range of research applications.

What is the advantage of using this kit over other silica-based methods for RNA extraction from fibrous tissues?

The NuAction™ Fibrous Tissue Kit offers superior RNA yields compared to other methods, ensuring high-quality RNA suitable for downstream applications.

Is the RNA purified with this kit compatible with microarray analysis?

Yes, the RNA extracted using this kit is suitable for microarray analysis, providing reliable and accurate results.

Can I automate the RNA purification process using the NuAction™ Fibrous Tissue Kit?

Yes, the kit can be conveniently automated on the Avam® AutEx™ platform, offering enhanced efficiency and reproducibility in RNA extraction from fiber-rich tissue samples.

Quantity

50 preps
SKU: NU8823 Category:
Updating…
Cart Close
  • No products in the cart.

Continue shopping