Price range: $134.00 through $500.00
Maximize the efficiency and reproducibility of your PCR experiments with the TaqBase™ PCR MasterMix Kit. Featuring TaqBase™ DNA Polymerase and the innovative OptiMix™ PCR Buffer, this kit minimizes the need for optimization, reduces pipetting steps, and ensures reliable PCR performance across a wide range of applications.
The TaqBase™ PCR MasterMix Kit offers researchers a streamlined solution for PCR experiments, delivering exceptional performance and convenience. This kit includes TaqBase™ DNA Polymerase, the unique OptiMix™ PCR Buffer, MgCl2, and ultrapure dNTPs, all optimized to provide reliable amplification with minimal optimization required.
Utilizing a premixed format, the TaqBase™ PCR MasterMix reduces pipetting errors and increases throughput, making it ideal for high-throughput applications. The OptiMix™ PCR Buffer, containing a balanced combination of KCI and (NH4)2SO4, ensures stringent primer-annealing conditions over a wide range of annealing temperatures and Mg2+ concentrations, minimizing the need for optimization.
Each lot of TaqBase™ DNA Polymerase undergoes comprehensive quality control tests, guaranteeing reproducible and specific amplification. With a storage stability of up to 2 months at 2-8°C, the master mix can be conveniently stored, further streamlining PCR setup.
For 250 Units
● TaqBase™ PCR MasterMix (250 units, 3 X 1.7mL)
● Distilled water (3X 1.7mL)
● Concentration of 5 units/µL
● 5’-3’ exonuclease acitivity
● 1000000 Fold amplification efficiency with a high specific to non-specific primer binding ratio.
● Polymerase extends at 2-4 kb/minute at 72°C
● Polymerase has the capability to add extra As.
● Each 20µL reaction requires 10µL of MasterMix and 10µL of DNA and primers in appropriate dilutions making a 1X PCR mix
Optimal for the following applications:
● DNA fingerprinting
● RT-PCR
● General PCR
● Screening
Yes, the 2X MasterMix format reduces pipetting steps, making it ideal for high-throughput experiments.
The master mix can be stored at 2-8°C for up to 2 months, providing long-term convenience and stability.
Optimization is minimized due to the unique OptiMix™ PCR Buffer, which provides stringent primer-annealing conditions over a wide range of parameters.
| Quantity | 1000 U, 250 U |
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