Streamline cfDNA library preparation with the VersaKit™ cfDNA, enabling sensitive variant detection from plasma to NGS libraries.
VersaKit™ cfDNA
Price range: $1,075.00 through $1,744.00
Purchase this item and get Product Description
The Versakit™ cfDNA offers a comprehensive solution for next-generation: sequencing (NGS)-based liquid biopsies, enabling optimal conversion of cell-free DNA (cfDNA) from plasma to NGS libraries. With highly efficient ligation chemistry, this kit supports a wide range of cfDNA inputs (1-100 ng) and allows direct library preparation without quantification. It facilitates PCR-free library generation from as little as 10 ng of cfDNA, minimizing PCR bias and reducing cross-contamination risk. The pre-dispensed plate-based adapters enable the processing of up to 96 samples per NGS run, enhancing workflow efficiency and reducing hands-on time. Whether you’re conducting cancer therapy monitoring, biomarker discovery research, or non-invasive prenatal testing, the VersaKit™ cfDNA provides sensitive detection of even the rarest variants, ensuring high confidence in your cfDNA findings.
Kit Contents
● Enzyme and buffer master mix for cfDNA library prep
● Adapter Plate 24-plex
● Library Amplification Primer
● PCR Master Mix
Technical Parameters
● Optimal Input Range: 1–100 ng cfDNA
● Library Type: PCR-free libraries
● Adapters: Pre-dispensed plate-based adapters for up to 96
samples/NGS run
● PCR Amplification: High-fidelity amplification reagents included for
<10 ng cfDNA
● Processing Time: < 3 hours for cfDNA extraction and library
preparation
● Multiplexing: Up to 96 samples for Illumina sequencers, up to 24
samples for Ion Torrent sequencers
Applications
The VersaKit™ cfDNA is suitable for any NGS-based cfDNA application,
including whole genome or hybrid capture sequencing for biomarker
discovery research, cancer therapy monitoring, and non-invasive prenatal
testing. It facilitates sensitive variant detection and provides highly reliable
results across a wide range of sample inputs.
Product FAQs
Can the VersaKit™ cfDNA be used for library preparation from plasma samples?
Yes, the kit is specifically designed for library preparation from plasma samples, enabling sensitive variant detection from cfDNA.
What is the input range supported by the VersaKit™ cfDNA?
The kit supports a wide input range of 1-100 ng cfDNA, allowing flexibility in sample preparation.
Does the kit include adapters for multiplexing samples?
Yes, the kit includes pre-dispensed plate-based adapters for multiplexing up to 96 samples for Illumina sequencers and up to 24 samples for lon Torrent sequencers.
Is quantification of cfDNA required before library preparation with this kit?
No, the kit allows direct library preparation without quantification, minimizing workflow complexity and reducing hands-on time.
| Reactions | Library Kit: 24 reactions, Universal Kit: 24 reactions |
|---|
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