VersaKit™ Bacterial/Fungal DNA/RNA/Protein offers a convenient solution for isolating high-purity plasmid or cosmid DNA, providing reproducible yields of molecular biology grade DNA suitable for various applications.
VersaKit™ Bacterial/Fungal DNA/RNA/Protein
Price range: $125.00 through $541.00
Purchase this item and get Product Description
The VersaKit™ Bacterial/Fungal DNA/RNA/Protein simplifies the process of isolating high-purity plasmid or cosmid DNA, offering a streamlined workflow for molecular biology applications. With its robust performance and reproducible yields, this kit provides researchers with reliable DNA purification for downstream experiments such as PCR, sequencing, and cloning.
Utilizing the VersaKit™ Spin Columns, the kit employs a bind-wash-elute procedure to efficiently capture DNA, removing impurities and contaminants in the process. The unique silica membrane within the columns binds DNA in the presence of a high concentration of chaotropic salt, allowing for elution in a small volume of low-salt buffer. This eliminates the need for time-consuming phenol-chloroform extraction and alcohol precipitation steps, ensuring the immediate availability of high-quality DNA for further analysis.
Moreover, the VersaKit™ Bacterial/Fungal DNA/RNA/Protein is designed for versatility and convenience. It offers a single protocol suitable for both high- and low-copy vectors, enabling researchers to obtain consistent results regardless of the vector type. Additionally, the kit includes GelNav loading dye, facilitating easy analysis of purified DNA samples on agarose gels.
Whether performing routine plasmid purification or demanding molecular biology experiments, the VersaKit™ Bacterial/Fungal DNA/RNA/Protein provides researchers with a reliable solution for obtaining high-purity DNA efficiently and reproducibly.
Kit Contents
● VersaKit™ Spin Columns (50)
● Reagents
● Buffers
● Collection Tubes (2 mL)
Technical Parameters
● Throughput: 1–24 samples
● Preparation Time: 24 minipreps in 30 minutes
● Lysate Clearing: Centrifugation
● Minimum Elution Buffer Volume: 50 µL
● Culture Volume for High-Copy Plasmids: 1–5 mL
● Culture Volume for Low-Copy Plasmids/Cosmids: 1–10 mL
Applications
● PCR
● Restriction digestion
● Ligation and transformation
● Sequencing
● Screening
Product FAQs
What is the composition of the VersaKit™ Bacterial/Fungal DNA/RNA/Protein?
The kit includes VersaKit™ Spin Columns, reagents, buffers, and collection tubes necessary for plasmid DNA purification.
How does the VersaKit™ Bacterial/Fungal DNA/RNA/Protein achieve high-purity DNA isolation?
VersaKit™ Spin Columns contain a unique silica membrane that binds DNA in the presence of a high concentration of chaotropic salt, allowing elution in a small volume of low-salt buffer, eliminating the need for phenol-chloroform extraction and alcohol precipitation.
Can the VersaKit™ Bacterial/Fungal DNA/RNA/Protein be automated?
Yes, the kit can be fully automated on the Avam® AutEx™, offering simplified handling and faster sample processing.
| Reactions | VersaKit™ Next Spin Kit: 100 reactions, VersaKit™ Spin Kit: 50 reactions, Zero Waste Kit: 50 reactions / $125, 250 reactions |
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