The NuAction™ FFPE DSP Kit is designed for efficient isolation of RNA from FFPE tissue sections, offering reliability and integrity preservation for downstream applications.
NuAction™ FFPE DSP Kit
$663.00
Purchase this item and get Product Description
The NuAction™ FFPE DSP Kit represents a breakthrough solution for researchers requiring high-quality RNA extraction from FFPE tissue sections. FFPE samples pose unique challenges due to formalin-induced crosslinking and fragmentation of nucleic acids. This kit addresses these challenges by incorporating advanced lysis and incubation conditions designed to reverse formaldehyde modifications of RNA, thus preserving RNA integrity.
Through the use of specialized lysis buffers and optimized protocols, the kit enables efficient release of RNA from FFPE tissue sections while minimizing further RNA degradation. Moreover, the inclusion of DNase and DNase Booster Buffer ensures thorough removal of genomic DNA contamination, enhancing the purity of the extracted RNA.
A key feature of the NuAction™ FFPE DSP Kit is its compatibility with downstream applications, offering researchers the flexibility to utilize purified RNA fragments in a wide range of molecular biology assays. While RNA from FFPE tissue sections may be fragmented due to fixation treatment, the kit’s robust purification process ensures that the RNA fragments obtained are suitable for various applications, including RT-PCR, qRT-PCR, and gene expression analysis.
The kit’s spin-column-based technology streamlines the RNA purification process, providing researchers with a reliable and convenient method for isolating RNA from FFPE samples. With the included Deparaffinization Solution, proteinase K, and NuAction™ Spin columns, users can achieve consistent and reproducible results, even from challenging FFPE samples.
Overall, the NuAction™ FFPE DSP Kit offers researchers a powerful tool for extracting high-quality RNA from FFPE tissue sections, enabling accurate and reliable downstream analysis. Whether performing gene expression studies, biomarker discovery, or molecular diagnostics, this kit ensures optimal RNA recovery and integrity, paving the way for groundbreaking research and diagnostic advancements in the field of molecular biology.
Kit Contents
● NuAction™ Spin Columns
● Collection Tubes
● Elution Tubes
● Deparaffinization Solution
● Avam® Proteinase K
● Avam® RNase-Free DNase I
● DNase Enhance Buffer
● RNase-Free Buffers
● Nuclease-Free Water
Technical Parameters
● Total RNA elution volume: 14–32 µl
● Recommended sample input: FFPE tissue sections
● RNA yield: Varies depending on sample and processing conditions
● RNA integrity: Preserved for downstream applications
● Spin-column based technology
Applications
● Isolation of total RNA from FFPE tissue sections
● Suitable for downstream applications requiring RNA fragments
● Note: RNA from FFPE tissue sections may be heavily fragmented due to fixation treatment, potentially impacting downstream applications requiring full-length RNA.
Product FAQs
Can I use the NuAction™ FFPE DSP Kit for in vitro diagnostic purposes?
Yes, the NuAction™ FFPE DSP Kit is intended for in vitro diagnostic use.
What is the elution volume for RNA purified using this kit?
RNA can be eluted in volumes ranging from 14 to 32 µl, depending on user preference and downstream application requirements.
Does the kit include components for genomic DNA removal?
Yes, the kit includes RNase-Free DNase I and DNase Booster Buffer for optimal removal of genomic DNA contamination.
What downstream applications are compatible with RNA isolated using this kit?
RNA purified using the NuAction™ FFPE DSP Kit is suitable for a wide range of downstream applications, including RT-PCR, qRT-PCR, and other molecular biology assays. However, please note that due to the fixation treatment, RNA from FFPE tissue sections may be heavily fragmented, potentially affecting applications requiring full-length RNA.
| Quantity | 50 preps |
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