Swiftly isolate highly pure total RNA, including small RNA fractions, from exosomes and extracellular vesicles with the NuAction™ Exosome Free Kits.
NuAction™ Exosome Free Kits
Price range: $876.00 through $3,105.00
Purchase this item and get Product Description
Uncover the genetic treasures concealed within exosomes and extracellular vesicles (EVs) using the NuAction™ Exosome Free Kits. These innovative kits utilize membrane affinity spin columns to efficiently capture exosomes from diverse biofluids, including urine, serum, plasma, cerebrospinal fluid (CSF), and cell culture supernatant. Once captured, total RNA, including the small RNA fraction, is isolated using proven NuAction™ Elution spin columns. The midi column format enables efficient processing of smaller sample volumes, while the maxi column format accommodates larger sample volumes, facilitating the detection of low-abundance RNAs with high sensitivity.
With a convenient on-column procedure, researchers can access purified RNA in just one hour, making the NuAction™ Exosome Free Kits ideal for time-sensitive experiments. The resulting RNA is free from non-vesicular protein complexes, ensuring exclusive isolation from exosomes and EVs. The purified RNA is highly suited for a variety of downstream applications, including transcriptomics, miRNA profiling, mRNA profiling, RNA sequencing. and more.
Whether you’re investigating biomarkers in urine-derived exosomes, studying the role of exosomal RNA in cancer progression, or exploring the therapeutic potential of EVs, these kits provide researchers with a reliable tool to unlock the molecular mysteries of extracellular vesicles.
The NuAction™ Serum/Plasma Kit includes both midi and maxi exoEasy columns, enabling analysis at varied volumes of prefiltered biofluids. The protocol for NuAction™ purification of exosomes and other extracellular vesicles not only offers faster processing than ultracentrifugation but also delivers cleaner preparations. Scanning electron microscopy demonstrates that NuAction™ elutes intact vesicles with higher purity compared to ultracentrifugation, ensuring superior results for your research endeavors.
Kit Contents
● 50 NuAction™ Exosomal Midi columns
● 50 NuAction™ spin columns
Technical Parameters
● Format: Silica spin filter tubes
● Throughput: 1-6 samples
● Time per run or per prep: 40 minutes
● Processing: Bead beating
● Storage temperature: Store lyophilized DNase I at 4°C. All other kit reagents and components should be stored at room temperature (15-30°C)
● Sample types: Urine, serum, plasma, cerebrospinal fluid (CSF), cell
culture supernatant
● Column type: Midi/Maxi
Applications
● Isolation of total RNA, including mRNA and miRNA, from exosomes and extracellular vesicles
● Transcriptomics
● miRNA profiling
● mRNA profiling
● RNA sequencing
Product FAQs
Can the NuAction™ Exosome Free Kits isolate RNA exclusively from exosomes?
Yes, these kits are specifically designed to isolate highly pure total RNA, including the small RNA fraction, exclusively from exosomes and extracellular vesicles, excluding RNA from non-vesicular protein complexes.
What sample types are compatible with the NuAction™ Kits?
The NuAction™ Kits are compatible with a wide range of biofluid samples, including urine, serum, plasma, cerebrospinal fluid (CSF), and cell culture supernatant.
How long does the RNA isolation process take with the NuAction™ Kits?
The entire RNA isolation process, from exosome purification to RNA extraction, can be completed in just one hour using the NuAction™ Exosome Free Kits, providing rapid results for time-sensitive experiments.
Can the NuAction™ Kits be automated?
Yes, the RNA isolation part of the protocol using NuAction™ Kits can be automated on the Avam® AutEx™ Platform, streamlining the workflow and increasing efficiency in the laboratory.
| Quantity | Maxi Kit:50 preps, Midi Kit: 50 preps, Midi/Maxi Kit: 20 preps |
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