OptiClean™ PCR Cleanup Kit

OptiClean™ PCR Cleanup Kit

$931.00

The OptiClean™ PCR Cleanup Kit provides a high-throughput solution for purifying DNA from PCR, sequencing, and enzyme reactions, ensuring highly pure PCR products suitable for downstream applications.

Purchase this item and get 93 Points
Purchase this item and get 93 Points
Product Description

The OptiClean™ PCR Cleanup Kit offers a streamlined and efficient solution for high-throughput purification of DNA derived from PCR, sequencing, and enzyme reactions. Designed for use in molecular biology laboratories, this kit simplifies the process of DNA cleanup, ensuring the production of highly pure PCR products suitable for a wide range of downstream applications. Utilizing silica spin filter plates, the OptiClean™ PCR Cleanup Kit effectively removes primers, dNTPs, enzymes, and other unwanted contaminants, resulting in purified DNA fragments within the size range of 60 bp to 10 kb. This comprehensive purification process eliminates primer-dimers of up to 50 bases, enhancing the yield recovery and purity of the final DNA product. Formulated to meet the needs of high-throughput workflows, this kit enables purification from up to 384 samples simultaneously, making it ideal for laboratories requiring efficient and reliable DNA cleanup methods for large-scale experiments. The ready-to-use nature of the kit, combined with its user-friendly protocol, ensures minimal hands-on time and rapid processing of samples, ultimately saving time and labor in the laboratory

Kit Contents

● Silica spin filter plates
● Binding buffer
● Wash buffer
● Elution buffer
● Collection plates

Technical Parameters​

● High-throughput DNA cleanup from PCR reactions
● Purification of sequencing products
● Cleanup of enzyme reaction products
● Removal of primer-dimers

Applications

Suitable for downstream applications such as RT-PCR, qPCR, and next-generation sequencing.

Product FAQs
Can this kit be used for purifying DNA fragments outside the specified size range?

The OptiClean™ PCR Cleanup Kit is optimized for DNA fragments ranging from 60 bp to 10 kb. While it may be possible to use the kit for purifying DNA fragments outside this range, optimal results are obtained within the specified size range. 

How does the kit ensure complete removal of primer-dimers?

The kit utilizes silica spin filter plates that efficiently capture and remove primer-dimers, labels, enzymes, and other unwanted reaction components. This purification method results in highly pure PCR products with minimal primer-dimer contamination.

Can the purified DNA be used directly in downstream applications such as sequencing or cloning?

Yes, the DNA purified using the OptiClean™ PCR Cleanup Kitis ready-to-use and suitable for a wide range of downstream applications, including sequencing, cloning, PCR, and more. The purified DNA is of high quality and purity, ensuring reliable results in subsequent experiments.

What is the throughput of this kit?

The kit is designed for high-throughput purification of DNA in a 96-well format, making it suitable for processing multiple samples simultaneously. With its efficient workflow and optimized protocol, researchers can purify DNA from up to 384 samples in a single run, saving time and labor.

Quantity

384 preps
SKU: NA6982 Category:
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