Facilitate the reliable isolation of DNA from challenging soil microbes using bead-based homogenization with the NuAction™ Soil Lysis Kit, designed for high-quality DNA extraction from diverse environmental samples.
NuAction™ Soil Lysis Kit
Price range: $428.00 through $802.00
Purchase this item and get Product Description
The NuAction™ Soil Lysis Kit is specifically designed for the rapid and efficient isolation of DNA from challenging soil microbes. With its optimized protocol and Inhibitor Cleanup Technology, this kit ensures high-quality, purified DNA suitable for downstream applications. Utilizing bead-based homogenization, this kit effectively lyses tough microbial cells present in various environmental samples, including compost, sediment, manure, and soil. The Inhibitor Cleanup Technology in the NuAction™ Soil Lysis Kit eliminates humic acid and other PCR inhibitors commonly found in soil samples, enabling successful qPCR amplification and detection of a wide range of microorganisms. This kit offers a short and simple protocol, allowing DNA isolation from up to 250 mg of soil samples in just 30 minutes. The purified DNA is ready for downstream applications, including next-generation sequencing, PCR, and genetic analysis.
Kit Contents
● Silica Spin Filter Tubes
● Buffers and reagents
● Glass beads (0.1 mm)
Technical Parameters
● Sample types: Processed soil, fecal, water, food, insects, swabs with
PCR inhibitors
● Processing: Bead beating
● Format: Silica Spin Filter Tubes
● Throughput: 1-24 samples
● Time per run or per prep: 30 minutes
● Binding capacity: Up to 20 µg per prep
● Storage temperature: Store at room temperature (15-30°C)
● Bead size: 0.1 mm glass
● Sample size: 0.25 g
Applications
● qPCR
● Next-generation sequencing
● Genetic analysis
● Microbial diversity studies
● Environmental microbiology
Product FAQs
Can this kit effectively isolate DNA from soil samples with high humic acid content?
Yes, the NuAction™ Soil Lysis Kit utilizes Inhibitor Cleanup Technology to efficiently remove humic acid and other PCR inhibitors commonly found in soil samples.
Is the protocol compatible with bead-based homogenizers?
This kit can also be used with several bead-based homogenizers for DNA isolation from tough soil microbes.
What downstream applications is the purified DNA suitable for?
The purified DNA can be used for various downstream applications, including qPCR, next-generation sequencing, and genetic analysis of microbial communities.
| Quantity | 100 preps, 50 preps. |
|---|
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