TaqSense™ DNA Polymerase Kit provides highly efficient DNA synthesis at extreme temperatures, offering increased sensitivity and versatility for various PCR applications.
TaqSense™ DNA Polymerase
$54.60
Purchase this item and get Product Description
TaqSense™ DNA Polymerase is a robust, recombinant Taq DNA polymerase isolated from Thermus aquaticus. Engineered for superior performance, the enzyme facilitates DNA synthesis at elevated temperatures, ensuring high yields with minimal enzyme input and little optimization. TaqSense™ generates DNA fragments up to 5 kb with a half-life of 45 minutes at 95°C, leaving poly-A overhangs for convenient downstream applications.
The enzyme catalyzes DNA synthesis in the 5′-3′ direction and exhibits 5′-3′ exonuclease activity without 3′-5′ exonuclease activity. Supplied with 10X TaqSense™ KCI and 10X TaqSense™ (NH4)2SO4 reaction buffers, these enzymes offer flexibility and efficiency across diverse PCR conditions.
Kit Contents
● TaqSense™ DNA Polymerase (5 U/µL)
● 10X TaqSense™ KCl Reaction Buffer
● 10X TaqSense™ (NH4)2SO4 Reaction Buffer
Technical Parameters
● Storage temperature: -20°C
● TaqSense™ DNA Polymerase Unit Definition: The amount required to incorporate 10 nmol of dNTPs into an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.
Applications
● Amplification of short and medium-size DNA sequences
● Routine, diagnostic, and multiplex PCR
● TA cloning
Product FAQs
How should I choose between 10x TaqSense™ KCI and 10x TaqSense™ (NH4)2SO4 Reaction Buffers?
10X TaqSense™ KCI buffer is recommended for applications requiring high specificity, while 10x TaqSense™ (NH4)2SO4 buffer is ideal for assays demanding high sensitivity and amplification efficiency, such as multiplex PCR. Both buffers can be evaluated to determine the most suitable option for specific applications.
Can TaqSense™ DNA Polymerase Kit be used for TA cloning?
Yes, TaqSense™ DNA Polymerase Kit is suitable for TA cloning, providing efficient amplification of DNA fragments with ‘A’ overhangs, facilitating seamless integration into TA cloning vectors.
| Quantity | 200U |
|---|
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