Achieve superior cDNA synthesis with the TaqBase™ Reverse Transcriptase Kit, featuring an RNase H-deficient M-MLV reverse transcriptase for robust and high-fidelity cDNA amplification.
TaqBase™ Reverse Transcriptase
$167.00
Purchase this item and get Product Description
TaqBase™ Reverse Transcriptase is an advanced tool for molecular biologists seeking superior cDNA synthesis performance. This innovative kit features a highly efficient Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase engineered with point mutations in the RNase H domain, effectively eliminating RNase H activity. Unlike traditional reverse transcriptases, TaqBase™ Reverse Transcriptase delivers exceptional thermostability, enhancing cDNA yield and integrity, especially for full-length transcripts from poly(A) mRNA or total RNA. With its optimized formulation, TaqBase™ Reverse Transcriptase unparalleled performance, making it the preferred choice for RT-PCR, cDNA cloning, and library construction for RNA-Seq applications.
Kit Contents
● 10,000 U of TaqBase™ Reverse Transcriptase (0.05 mL at 200,000
U/mL)
● 5X Reverse Transcriptase Buffer (1 × 1.5 mL)
● 100 mM DTT (1 × 1.5 mL)
Technical Parameters
● Optimum extension temperature: 42°C
● Transcript length: Up to 9.4 kb
Applications
● RT-PCR amplification
● cDNA cloning
● Library construction for RNA-Seq
Product FAQs
What is the advantage of using TaqBase™ Reverse Transcriptase?
TaqBase™ Reverse Transcriptase offers increased thermal stability and lacks RNase H activity, resulting in enhanced cDNA yield and integrity.
Can TaqBase™ Reverse Transcriptase be used for RT-PCR?
Yes, TaqBase™ Reverse Transcriptase is suitable for RT-PCR amplification of RNA targets.
What is the transcript length capability of TaqBase™ Reverse Transcriptase?
TaqBase™ Reverse Transcriptase can efficiently synthesize cDNA transcripts up to 9.4 kb in length.
| Quantity | 10000U |
|---|
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