The TaqBase™ II DNA polymerase is a thermally stable, processive DNA polymerase suitable for routine PCR amplification of DNA fragments up to 5 kb.
TaqBase™ II DNA Polymerase
$474.00
Purchase this item and get Product Description
TaqBase™ II DNA Polymerase provides a thermally stable, processive 5′→3′ DNA polymerase suitable for routine PCR amplification of DNA fragments up to 5 kb. Derived from the thermophilic organism Thermus aquaticus YT-1, this 94 kDa polymerase possesses an inherent 5′→3′ exonuclease activity and lacks a 3′→5′ proofreading function. It excels at amplifying shorter
sequences from low complexity template sources and produces robust yields with minimal optimization of reaction conditions. The enzyme is supplied in a buffer containing Tris-HCl, NaCl, DTT, EDTA, stabilizer, and glycerol, while the accompanying 10X PCR Buffer provides optimal reaction conditions. TaqBase™ II DNA Polymerase is ideal for various molecular biology applications, including routine PCR amplification, in vitro amplification, cloning, and genotyping.
Kit Contents
● TaqBase™ II DNA Polymerase (10,000 U)
● 10X PCR Buffer (1 X 1.5 mL)
Technical Parameters
● Purity: >99%
● Specific activity: 74,625 U/mg
● Single-stranded exonuclease: Functional
● Double-stranded exonuclease: Functional
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● Storage temperature: –25°C to –15°C
● Molecular weight: 94 kDa
Applications
TaqBase™ II DNA Polymerase is suitable for a wide range of molecular biology applications, including:
● Routine PCR amplification of DNA fragments up to 5 kb
● In vitro amplification
● Cloning and genotyping
Product FAQs
What is the specific activity of TaqBase™ II DNA Polymerase?
The specific activity of TaqBase™ II DNA Polymerase is 74,625 U/mg.
Can TaqBase™ II DNA Polymerase amplify longer DNA fragments?
TaqBase™ II DNA Polymerase is optimized for amplifying DNA fragments up to 5 kb in length.
Is TaqBase™ II DNA Polymerase suitable for genotyping applications?
Yes, TaqBase™ II DNA Polymerase is suitable for genotyping applications due to its high specificity and efficiency in PCR amplification.
| Quantity | 10000U |
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