The SparkTaq™ MasterMix Kit (500) and the SparkTaq™ PCR Foundation Kit provide a format for a hot-start PCR that is known to be highly sensitive. It utilizes both DNA and cDNA templates and enables various features like visual pipetting control, tracking dyes for gel loading as well as providing stability at room temperature during and after the reaction. It gives a 4X concentrated master mix that enables a higher volume of input sample.
SparkTaq™ MasterMix and PCR Foundation Kits
Price range: $219.00 through $503.00
Purchase this item and get Product Description
The SparkTaq™ MasterMix Kit and SparkTaq™ PCR Foundation Kit deliver a highly sensitive hot-start PCR format suitable for both DNA and cDNA templates. Offering a 4X concentrated master mix, these kits enable increased input sample volumes, enhancing sensitivity for diverse targets. The hot-start mechanism ensures specificity by eliminating nonspecific amplification from the first cycle, guaranteeing reproducible results. The innovative formulations include separate vials of orange and blue tracers, allowing optional visual tracking during setup, while the adaptable PCR Buffer composition enables ultra-fast cycling conditions and stringent primer-annealing over a broad temperature range without the need for optimization. Guard protection enhances specificity and sensitivity, enabling detection down to a single target molecule. The optional tracers facilitate correct pipetting and efficient gel loading, enhancing workflow efficiency. With stable reactions at room temperature during and after setup, the SparkTaq™ MasterMix Kit provides a versatile solution for PCR with ultrafast cycling and up to 6 months of storage at 2-8°C, making it an ideal choice for robust and efficient PCR performance across various applications.
Kit Contents
SparkTaq™ MasterMix Kit
For 500 reactions
● 4X SparkTaq™ Master Mix (2 X 1.25 mL)
● 25X Template Guide (1X 0.2 mL)
● 125X MasterMix Guide (2X 0.05 mL)
● Nuclease-Free Water
SparkTaq™ PCR Foundation Kit
For 250 reactions
● 5U/µL SparkTaq™ DNA Polymerase (1 X 0.05 mL)
● 5X SparkTaq™ PCR Buffer (2 X 1.2 mL)
● 25X Template Guide (1X 0.2 mL)
● 125X MasterMix Guide (2X 0.05mL)
● 5X AVBlend™ (1 X 2 mL)
● 25mM MgCl2 (1 X 1.2 mL)
● 10mM dNTP Mix (1 X 0.055 mL)
● Nuclease-Free Water
Technical Parameters
● Reaction Buffer: Optimized PCR Buffer, ensuring robust amplification across various templates.
● Amplicon Length: Capable of amplifying up to 5 kb from genomic DNA.
● Annealing Temperature: Broad range, adjustable depending on primer design.
● Amplification Speed: High-speed PCR with short cycling times.
● Sensitivity: Detects low-copy targets with high sensitivity.
● Compatibility: Compatible with standard and fast PCR cycling protocols.
● DNA Input: Works efficiently with a wide range of DNA inputs, from low to high concentrations.
● Inhibitor Resistance: Tolerates common PCR inhibitors found in clinical or environmental samples.
Applications
Applicable for any template to be used for genotyping or genetic analysis with multiple primer pairs.
Product FAQs
Can the SparkTaq™ MasterMix Kit be used for both DNA and CDNA templates, and what is the recommended input volume?
Yes, the kit can be used for both DNA and cDNA templates with a high template input volume.
What are the advantages of the 4X concentration in the SparkTaq™ MasterMix and how does it enhance sensitivity?
The 4X concentration master mix allows for greater flexibility in input DNA volumes and concentrations.
How does the SparkTaq™ PCR Foundation Kit facilitate protocol optimization for specific PCR targets?
The individual components packaged in the Foundation kit allow the user to optimize and modify protocols according to the needs of input template to get high quality, reproducible yields.
Can the SparkTaq™ MasterMix and PCR Buffer accommodate ultra-fast cycling conditions?
Yes, the master mix and the specially formulated buffer allow for ultra-fast cycling conditions.
| Quantity | SparkTaq™ MasterMix Kit (500), SparkTaq™ PCR Foundation Kit (250) |
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