The Avam® Tth DNA Ligase is a robust molecular biology tool designed for high-efficiency ligation reactions, enabling various applications, including Ligase Chain Reaction (LCR), Ligase Detection Reaction (LDR), and Next-Generation DNA Sequencing (NGS).
Avam® Tth DNA Ligase
Price range: $119.00 through $774.00
Purchase this item and get Product Description
The Avam® Tth DNA Ligase provides a reliable solution for DNA ligation applications in molecular biology. Derived from a plasmid carrying the Thermus thermophilus DNA ligase gene, this enzyme exhibits remarkable thermostability, enabling efficient ligation reactions under high-stringency conditions. It catalyzes the formation of phosphodiester bonds between adjacent 3′-hydroxyl and 5′-phosphate termini in double-stranded DNA, utilizing NAD as a cofactor. With its high specificity, the Tth DNA Ligase selectively acts on double-stranded DNA, ensuring minimal interference with other nucleic acid species such as single-stranded DNA, RNA, or blunt-ended DNA.
Supplied with a meticulously formulated 10x Tth Ligation Buffer, the ensures optimal enzyme performance across a broad range of experimental conditions. The buffer composition includes essential components such as Tris-HCl, NaCl, EDTA, Triton X-100, DTT, and glycerol, providing an ideal environment for efficient ligation reactions. The Avam® Tth DNA Ligase is supplied in a convenient format, offering flexibility for various experimental setups and applications.
With its robust performance and versatility, the Avam® Tth DNA Ligase is an indispensable tool for a wide range of molecular biology applications, including Ligase Chain Reaction (LCR), Ligase Detection Reaction (LDR), Next-Generation DNA Sequencing (NGS), Repeat Expansion Detection (RED), Rolling Circle Amplification (RCA), and Proximity Ligation Assay (PLA). Whether conducting routine laboratory experiments or advanced molecular techniques, this delivers consistent and reliable results, making it an essential component of any molecular biology toolkit.
Kit Contents
● Avam® Tth DNA Ligase: 50 μL at 5 U/μL
● 10x Tth Ligation Buffer: 125 μL
Technical Parameters
● Storage temperature: -20°C
● Thaw cycles for buffer: Up to 20 cycles without compromising
performance
Applications
● Ligase Chain Reaction (LCR)
● Ligase Detection Reaction (LDR)
● Next-Generation DNA Sequencing (NGS)
● Repeat Expansion Detection (RED)
● Rolling Circle Amplification (RCA)
● Proximity Ligation Assay (PLA)
Product FAQs
What is the storage temperature for the Avam® Tth DNA Ligase?
The enzyme should be stored at -20°C to maintain enzyme stability.
How many thaw cycles can the 10x Tth Ligation Buffer withstand?
The 10x Tth Ligation Buffer can withstand up to 20 thaw cycles without compromising its performance.
What is the specific activity of the Avam® Tth DNA Ligase?
One unit of Avam® Tth DNA Ligase catalyzes the ligation of 50% of the cos sites present in 1 µg of bacteriophage lambda DNA in 1 minute at 45°C.
Can the Avam® Tth DNA Ligase be used for blunt-ended DNA ligation?
No, the Avam® Tth DNA Ligase is not active against blunt-ended DNA substrates.
| Quantity | 2500U, 5000U, 500U |
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