Enhance your molecular biology experiments with the Avam® Thermostable Pyrophosphatase, featuring a highly stable enzyme for efficient orthophosphate production.
Avam® Thermostable Pyrophosphatase
$401.00
Purchase this item and get Product Description
The Avam® Thermostable Pyrophosphatase offers a reliable solution for hydrolyzing inorganic pyrophosphate into orthophosphate, essential for various molecular biology applications. This pack includes 250 U of a recombinant enzyme sourced from Sulfolobus acidocaldarius, known for its robust activity and remarkable thermostability. With an optimal temperature for activity at 75°C and a low affinity constant of 5.4 µM for pyrophosphate, this enzyme ensures precise and efficient performance across a broad pH range. It is suitable for avoiding inhibition in RNA synthesis, modification reactions, and PCR, providing researchers with consistent and reproducible results.
Kit Contents
● 250 U of Avam® Thermostable Pyrophosphatase (1X 0.625 mL at
2,000 U/mL)
● 10X Tth Ligation Buffer (1 X 125 μL)
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 19.4 kilo Daltons
● Specific activity: >95%
● Single-stranded exonuclease: <1.0% released
● Double-stranded exonuclease: <1.0% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● Production of orthophosphate
● Avoiding inhibition in RNA synthesis and modification reactions
● Avoiding inhibition in PCR reactions
Product FAQs
What is the optimal temperature for the activity of Avam® Thermostable Pyrophosphatase?
The optimal temperature for activity is 75°C.
Can Avam® Thermostable Pyrophosphatase be used under PCR conditions?
Yes, it is functional under PCR conditions.
What is the principle behind the Avam® Thermostable Pyrophosphatase assay?
One unit of the enzyme liberates 1 µmol of phosphate per minute from inorganic pyrophosphate at 75°C and pH 8.5.
| Quantity | 1250U |
|---|
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