Efficiently prevent carryover contamination in your PCR experiments with Avam® Thermolabile UDG, featuring a highly active enzyme that removes uracil from DNA.
Avam® Thermolabile UDG
$401.00
Purchase this item and get Product Description
Avam® Thermolabile UDG is a potent enzymatic solution designed for molecular biology applications, particularly for preventing carryover contamination in PCR reactions. This enzyme effectively removes uracil from DNA by hydrolyzing the N-glycosylic bond, leaving behind an apurinic or apyrimidinic site. Notably, it exhibits no activity on RNA substrates, making it suitable for RT-PCR. The enzyme’s thermolability ensures complete inactivation with a simple 10-minute incubation at temperatures exceeding 50°C, ensuring precise control over experimental conditions. Supplied with a convenient 10x Reaction Buffer, this enzyme offers researchers a reliable tool for achieving accurate and reproducible results in their molecular biology experiments.
Kit Contents
● 500 U of Avam® Thermolabile UDG (0.5 mL at 1000 U/mL)
● 10X Thermolabile UDG Reaction Buffer (2 x 1.5 mL)
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 26.2 Daltons
Applications
This product is suitable for various molecular biology applications, including:
● Carryover prevention in PCR
● Removal of uracil from DNA substrates
Product FAQs
Can this enzyme be used for RT-PCR?
Yes, Avam® Thermolabile UDG is suitable for RT-PCR as it exhibits no activity on RNA substrates.
How can I completely inactivate the enzyme?
The enzyme can be irreversibly inactivated by incubating at temperatures greater than 50°C for 10 minutes.
Is it necessary to exchange buffers when using this enzyme?
No, Avam® Thermolabile UDG is active in most molecular biology reaction buffers, eliminating the need for buffer exchange.
What is the shelf life of this product?
The shelf life is indicated on the product label and should be followed for optimal performance.
| Quantity | 1250U |
|---|
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