Expertly extend your DNA and RNA templates and label 3’ ends with the incorporation of dATP and dTTP with high efficiency using the Avam® Terminal Deoxynucleotidyl Transferase.
Avam® Terminal Deoxynucleotidyl Transferase
$443.00
Purchase this item and get Product Description
The Avam® Terminal Deoxynucleotidyl Transferase (TdT) provides the necessary components for efficient extension of DNA and RNA, useful for 3′ labeling and incorporation of dATP and dTTP with high efficiency. Avam® Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase used to add deoxynucleotides to the 3′ hydroxyl terminus of single or double-stranded DNA molecules. This pack includes 6000 U of TdT enzyme, 10X VividGreen™, and 2.5 mM Cobalt Chloride, facilitating the non-templated addition of dNTPs to 3′ termini of DNA. The presence of 1 mM Co2+ stimulates the tailing of the 3′-ends of DNA fragments. The enzyme efficiently extends blunt, 5′ overhanging, and single-stranded DNA and RNA. With its ability to incorporate dATP and dTTP with a fivefold higher efficiency than dCTP and dGTP, TdT is invaluable for various molecular biology applications requiring efficient DNA and RNA extension. It enables researchers to label 3′ ends with modified nucleotides, add homopolymeric tails to 3′-OH DNA ends, and label blunt ends of dsDNA breaks, as in the TUNEL assay.
Kit Contents
● Avam® Terminal Deoxynucleotidyl Transferase (TdT) enzyme (6000
U)
● 10X VividGreen™ (1 X 1.5 mL)
● 2.5 mM Cobalt Chloride (1 X 1.5 mL)
Technical Parameters
● Purity: >99%
● Specific Activity: 27,400 U/mg
● Single-stranded exonuclease: <5.0% released
● Double-stranded exonuclease: <1.0% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● Storage temperature: –25°C to –15°C
● Molecular weight: 82.6 kDa
● One unit defined as the amount of polymerase required to convert 1
nmol of dTTPs into acid-insoluble material in 1 hour at 37°C
Applications
The Avam® Terminal Deoxynucleotidyl Transferase is suitable for various
molecular biology applications, including:
● Labeling of 3ʹ ends with modified nucleotides
● Adding homopolymeric tails to 3’-OH DNA ends
● Labeling blunt ends of dsDNA breaks (TUNEL assay)
Product FAQs
What is the specific activity of the TdT enzyme?
The TdT enzyme has a specific activity of 27,400 U/mg.
Can the TdT enzyme incorporate ribonucleotides or modified nucleotides?
Yes, TdT can incorporate ribonucleotides, biotins, and dideoxynucleotides in the presence of Co2+.
How is the activity of the TdT enzyme measured?
The activity of the TdT enzyme is measured by converting dTTPs into acid-insoluble material in a specified time frame at 37°C.
Is the TdT enzyme compatible with various DNA substrates?
Yes, the TdT enzyme efficiently extends blunt, 5′ overhanging, and single-stranded DNA and RNA substrates.
| Quantity | 6000U |
|---|
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