The Avam® Large DNA Construct Kit offers a simple and efficient solution for the purification of transfection-grade large-molecular-weight DNA, ensuring genomic DNA contamination removal and yielding pure DNA suitable for various molecular biology applications.
Avam® Large DNA Construct Kit
$583.00
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The Avam® Large DNA Construct Kit presents gravity-flow, anion-exchange columns designed for the purification of large-molecular-weight DNA with exceptional efficiency. Featuring an integrated ATP-dependent exonuclease digestion step, this kit ensures the selective removal of contaminating genomic DNA, enhancing the purity of the final DNA product. The purified DNA obtained through this kit is equivalent to that achieved by 2X CsCI gradient centrifugation and is ideal for transfection applications. The performance of the Avam® Large DNA Construct Kit is highlighted by its ability to yield DNA that is free from genomic DNA contamination, as depicted in the figure showcasing the efficient removal of genomic DNA. This purity enables accurate DNA quantitation, facilitating sensitive experiments conducted under defined and reproducible conditions. Utilizing an optimized gravity-flow procedure, the kit’s anion-exchange resin provides DNA purity comparable or superior to that obtained through CsCI gradient centrifugation. Prepacked Avam® AnioFlow tips operate via gravity flow, minimizing hands-on time and eliminating the need for toxic substances like phenol, chloroform, and CsCI, ensuring user and environmental safety. The procedure involves alkaline lysis of up to 500 mL culture, followed by an integrated digestion step with ATP-dependent exonuclease for selective removal of contaminants. Subsequently, the sample is loaded onto the anion-exchange tip, where plasmid DNA binds selectively. After impurities removal and elution in high-salt buffer, the DNA is concentrated and desalted, ensuring pure plasmid DNA suitable for various applications. The DNA purified using the Avam® Large DNA Construct Kit finds utility in a wide range of applications, including subcloning, shotgun library preparation, direct sequencing of large-construct DNA, and transfection, making it a versatile tool in molecular biology research.
Kit Contents
● Avam® Flow Tips (10 tips)
● Reagents
● Buffers
● ATP-Dependent Exonuclease
Technical Parameters
● Sample Source: Whole blood, cultured cells, tissue, yeast,
Gram-negative bacteria, Gram-positive bacteria
● Amount: Varies based on sample source
● Yield: Varies based on sample source
● Format: Anion-exchange tips
● Processing: Gravity-flow, bead beating
● Sample Size: Up to 500 mL culture
Applications
● Subcloning
● Shotgun library preparation
● Direct sequencing of large-construct DNA
● Transfection
Product FAQs
Can the Avam® Large DNA Construct Kit be used for purification of DNA from bacterial cultures?
Yes, the kit is suitable for the purification of DNA from various sample sources, including bacterial cultures such as Gram-negative and Gram-positive bacteria.
Is the DNA purified using this kit free from contaminants such as RNA and proteins?
Yes, the DNA purified using the Avam® Large DNA Construct Kit is free from contaminants such as RNA, proteins, and metabolites, ensuring high-quality DNA suitable for downstream applications.
How long does the purification procedure take with the Avam® Large DNA Construct Kit?
The purification procedure typically takes around 1.5 to 2 hours, depending on the sample type and processing conditions.
Can the purified DNA be used for long-read sequencing applications?
Yes, the high-molecular-weight DNA purified using this kit is suitable for long-read sequencing applications, ensuring accurate and reliable sequencing results.
Is there a limit to the size of DNA fragments that can be purified using this kit?
The Avam® Large DNA Construct Kit allows for the purification of large-molecular-weight DNA fragments up to 150 kb in size, making it suitable for a wide range of molecular biology applications.
| Quantity | 10 preparations |
|---|
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