Concentrate and purify free-circulating DNA and RNA from plasma for in vitro diagnostic use with the DSP Circulating NA VersaKit™.
DSP Circulating NA VersaKit™
$1,632.00
Purchase this item and get Product Description
The DSP Circulating NA VersaKit™ enables efficient concentration and purification of free-circulating DNA and RNA from plasma samples, specifically designed for in vitro diagnostic use. With its streamlined protocol and omission of phenol-chloroform extractions, the kit simplifies the isolation process while ensuring high-quality nucleic acids. Utilizing Mini columns, the kit selectively binds nucleic acids, while contaminants are effectively removed in the wash steps. The inclusion of tube extenders and support for vacuum processing enhances flexibility in sample and elution volumes. Offering both Classic and Breeze protocols, the kit caters to different workflow preferences, providing options for efficient and low hands-on processing.
Kit Contents
● Mini columns
● Buffers
● Carrier RNA
● Avam® Proteinase K
● Elution tubes.
Technical Parameters
● Starting Sample Volume: Up to 5 mL
● Elution Volume: 20 µl to 150 µl
● Sample Types: Human plasma
● Concentration Range: 1–100 ng/ml circulating DNA in human plasma
Applications
● Purified circulating cell-free DNA (ccfDNA) from the kit is suitable for a
wide range of downstream applications, including next-generation
sequencing (NGS), reverse transcription PCR (RT-PCR), digital PCR,
multiplex PCR, and quantitative PCR.
Product FAQs
Is mechanical homogenization required for sample preparation with this kit?
No, mechanical homogenization is not necessary as the tissues are lysed enzymatically, simplifying the sample preparation process.
Can the elution volume be adjusted for specific downstream applications?
Yes, the elution volume is flexible and can be adjusted according to the requirements of downstream applications, ranging from 20–150 µl.
What are the typical concentrations of circulating DNA in human plasma?
The concentration of circulating DNA in human plasma typically ranges from 1–100 ng/mL.
| Quantity | 50 preps |
|---|
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