The OptiScript™ Whole Transcriptome Kit offers a streamlined solution for preamplification and reverse transcription of limited RNA samples to high cDNA yields, facilitating unlimited gene expression analysis via real-time PCR. With optimized reagents and a fast protocol, this kit ensures equal amplification of all transcript regions, enabling reliable and reproducible results.
OptiScript™ Whole Transcriptome Kit
Price range: $1,334.00 through $4,415.00
Purchase this item and get Product Description
The OptiScript™ Whole Transcriptome Kit stands at the forefront of molecular biology solutions, offering a transformative approach to transcriptome analysis. Designed to address the challenges posed by limited RNA samples, this kit embodies innovation and efficiency, enabling researchers to unlock the full potential of their experimental material. By integrating cutting-edge Φ29 polymerase technology with optimized reagents, the kit delivers unparalleled performance, consistently generating high cDNA yields from minimal starting material. This transformative capability not only streamlines experimental workflows but also expands the scope of gene expression studies, facilitating comprehensive analyses of complex biological systems. Moreover, the OptiScript™ Whole Transcriptome Kit offers more than just amplification; it represents a gateway to deeper insights into gene expression dynamics. Through its ability to uniformly amplify all transcript regions, the kit ensures an accurate reflection of the transcriptome landscape. This level of fidelity is crucial for unraveling the intricacies of gene regulation and uncovering subtle variations in expression profiles. Researchers can trust in the reliability and reproducibility of their results, confident in the kit’s capacity to faithfully preserve the transcript profile across samples and conditions. Beyond its technical prowess, the OptiScript™ Whole Transcriptome Kit embodies a commitment to user convenience and flexibility. With a fast and straightforward protocol, researchers can expedite their experiments without sacrificing data quality. Whether analyzing bulk RNA populations or studying rare cell populations, this kit empowers researchers to extract meaningful insights from diverse sample types. Furthermore, its compatibility with downstream applications such as real-time PCR ensures seamless integration into existing workflows, facilitating a seamless transition from sample preparation to data analysis. In essence, the OptiScript™ Whole Transcriptome Kit represents a paradigm shift in transcriptome analysis, empowering researchers to delve deeper into the complexities of gene expression regulation. With its robust performance, user-friendly design, and broad applicability, this kit sets a new standard for transcriptome analysis, enabling groundbreaking discoveries in molecular biology and beyond.
Kit Contents
● OptiScript™ Enzyme (1 X 0.1 mL)
● OptiScript™ Buffer (1 X 0.4 mL)
● Ligation Enzyme 1 (1 X 0.1 mL)
● Ligation Enzyme 2 (1 X 0.1 mL)
● Ligation Reagent (1 X 0.2 mL)
● Ligation Buffer (1 X 0.6 mL)
● OptiScript™ Midi DNA Polymerase (1 X 0.1 mL)
● OptiScript™ Midi Reaction Buffer (2 x 1.45 mL)
Technical Parameters
● RNA Input: From as little as 1 ng RNA
● cDNA Yield: Up to 40 μg
● Amplification Time: 2 hours or 8 hours
● Storage Conditions: Store components at recommended temperatures
Applications
● Real-time PCR analysis of gene expression
● Archiving cDNA for future studies
Product FAQs
What is the RNA input requirement for this kit?
The kit is optimized for use with as little as 1 ng of RNA, allowing for gene expression analysis from limited sample amounts.
How much cDNA can be generated using this kit?
Up to 40 μg of cDNA can be obtained from a single reaction, enabling extensive real-time PCR analyses.
Is the cDNA generated suitable for archiving?
Yes, cDNA prepared using this kit is stable and can be archived for future studies, providing a valuable resource for ongoing research.
Can this kit be used for microarray analysis?
No, the cDNA generated is not suitable for microarray analysis.
What is the amplification technology used in this kit?
The kit utilizes Multiple Displacement Amplification (MDA) technology with Φ29 polymerase for non-biased sequence amplification, ensuring uniform representation of all transcript regions.
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