Experience rapid cDNA synthesis and genomic DNA removal with the OptiScript™ Reverse transcription kit in just 20 minutes, ensuring efficiency and time-saving convenience. Yield high-quality cDNA, even from low-abundance transcripts, facilitating comprehensive gene expression analysis. Capture transcript sequences from both 5′ and 3′ regions, providing a comprehensive view of gene expression dynamics. Streamline your workflow while simplifying experimental design and execution with the brand-new OptiScript™ Reverse Transcription Kit.
OptiScript™ Reverse Transcription Kit
Price range: $524.00 through $7,916.00
Purchase this item and get Product Description
The OptiScript™ Reverse Transcription Kit offers a swift and efficient procedure for cDNA synthesis, seamlessly integrated with genomic DNA removal. The unique DNAPurge Buffer swiftly eliminates genomic DNA contamination from RNA samples, sparing the need for a separate DNase digestion step. With components like OptiScript™ Reverse Transcriptase, OptiScript™ RT Buffer, and a specialized RT Primer Mix, the kit ensures robust reverse transcription. This results in high cDNA yields suitable for real-time PCR analysis, covering all regions of mRNA transcripts. Utilizing the OptiScript™ Reverse Transcription Kit ensures the accurate removal of genomic DNA from RNA samples, as demonstrated by the effective action of the DNAPurge Buffer. This eliminates the need for additional DNase digestion steps, saving time and reducing costs. The high RNA affinity of GenoScript™ Reverse Transcriptase, in conjunction with OptiScript™ RT Buffer, facilitates efficient cDNA synthesis from a broad range of RNA amounts, spanning from 10 pg to 1 µg. Furthermore, the RT Primer Mix enables synthesis from all regions of RNA transcripts, including the challenging 5′ regions, ensuring comprehensive coverage for downstream applications. Notably, the kit provides superior sensitivity in real-time PCR, even for low-abundance genes, enhancing reproducibility in gene expression assays. The blend of ScriptSwift™ and VeraScript™ Reverse Transcriptases in the OptiScript™ Reverse Transcriptase Kit offers high affinity for RNA and robust performance across various RNA templates. The optimized OptiScript™ RT Buffer is compatible with real-time PCR buffer, streamlining the workflow for accurate and reliable results. The entire process of genomic DNA removal and cDNA synthesis is completed within a swift 20-minute timeframe, facilitated by a user-friendly procedure with consistent incubation temperatures and master mixes.
Kit Contents
For 50 reactions:
7X DNAPurge™ Buffer (1 X 0.1mL)
OptiScript™ Reverse Transcriptase (1 X 0.05mL)
5X OptiScript™ RT Buffer (1 X 0.2mL)
RT Primer Mix (1 X 0.05mL)
Nuclease-Free Water (1 X 1.9mL)
Technical Parameters
cDNA Synthesis Time: 20 minutes
cDNA Yield: High, even from low-abundance transcripts
Target Coverage: Includes 5′ and 3′ regions of transcripts
Genomic DNA Removal: Effective with gDNA Wipeout Buffer
Compatibility: Optimized for real-time PCR instruments
RNA Template Compatibility: 10 pg to 1 µg RNA
Sensitivity and Specificity: High for accurate quantification
Optimized Buffer System: Promotes efficient reverse transcription
Storage Stability: Shelf life of 12 months at -20°C
Ease of Use: Streamlined protocol and master mix format
Applications
The OptiScript™ Reverse Transcription Kit is suitable for highly efficient and sensitive qRT-PCR across various starting materials, including laser-microdissected samples and tissue biopsies research.
Product FAQs
What is the shelf life of the OptiScript™ Reverse Transcription Kit?
The OptiScript™ Reverse Transcription Kit has a shelf life of 12 months when stored properly at -20°C. Ensure that all components are kept tightly sealed to prevent degradation.
Can the OptiScript™ Reverse Transcription Kit be used with RNA samples containing inhibitors?
To improve performance, it is recommended to purify your RNA samples using methods that reduce the presence of such contaminants before using the OptiScript™ kit. If inhibitors are a concern, further optimization of your sample preparation protocols might be necessary to ensure the highest efficiency in your reverse transcription reactions. For specific handling and potential troubleshooting regarding inhibitors, consult the kit’s manual or the manufacturer for detailed guidance.
Can the synthesized cDNA be stored for future use?
Yes, the synthesized cDNA can be stored at -20°C for several months. It is advisable to aliquot the cDNA to avoid repeated freeze-thaw cycles.
Is the OptiScript™ Reverse Transcription Kit compatible with various real-time PCR instruments?
Yes, the synthesized cDNA is compatible with most real-time PCR instruments. However, it is recommended to validate the compatibility with your specific instrument and assay conditions.
Can the OptiScript™ Reverse Transcription Kit be used for gene expression analysis in single cells?
Yes, the kit offers a protocol for direct amplification from cultured cells, including single cells. However, it is important to optimize the protocol for single-cell analysis to ensure accurate results.
Does the kit include a positive control for reverse transcription efficiency?
No, the kit does not include a specific positive control for reverse transcription efficiency. However, the synthesized cDNA can be validated using appropriate positive controls in downstream PCR reactions.
| Reactions | 200, 2000, 400, 50 |
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