Facilitate accurate and efficient DNA synthesis with the Avam® T7 DNA Polymerase, featuring a highly processive polymerase with potent 3′→5′ exonuclease activity, ideal for various molecular biology applications.
Avam® T7 DNA Polymerase
$474.00
Purchase this item and get Product Description
Elevate your molecular biology experiments with the Avam® T7 DNA Polymerase, harnessing the power of Avam® T7 DNA Polymerase, a mesophilic, highly processive enzyme derived from bacteriophage T7. This replicative DNA polymerase plays a pivotal role in the rapid and precise replication of the phage’s genome during its infection cycle. Comprising a polymerase domain from gene 5 of T7 bacteriophage and a processivity factor from the E. coli trxA gene thioredoxin, this two-subunit protein offers exceptional fidelity and efficiency.
Avam® T7 DNA Polymerase boasts a robust (3′-5′) exonuclease activity that acts on both single and double-stranded DNA, contributing to its high fidelity and preventing strand displacement synthesis. The polymerase provides the enzyme in a buffer optimized for stability and activity, ensuring reliable performance in your experiments. The 10X Avam® T7 DNA Polymerase Buffer further enhances enzyme efficiency, facilitating seamless DNA synthesis and replication.
Kit Contents
● Avam® T7 DNA Polymerase (3500U, 0.35 mL)
● 10X DNA Polymerase Buffer (1 X 1.5 mL)
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 92.1 Daltons
● Purity: >99%
● Specific activity: 13,333 U/mg
● Single-stranded exonuclease: Functional
● Double-stranded exonuclease: Functional
● Double-stranded endonuclease: 100 U, no conversion
● E. coli DNA contamination: <10 copies
Applications
● Copying long stretches
● DNA manipulation
● Site-directed mutagenesis
● Second strand synthesis
● Gap filling reaction (no strand displacement)
● ISEL assay: apoptosis detection by in situ labeling of DNA breaks
Product FAQs
Is Avam® T7 DNA Polymerase suitable for DNA sequencing?
No, Avam® T7 DNA Polymerase is not suitable for DNA sequencing due to its exonuclease activity.
What is the recommended DNA molar ratio for DNA synthesis using Avam® T7 DNA Polymerase?
The recommended DNA molar ratio is dependent on the specific application and should be optimized accordingly
Can Avam® T7 DNA Polymerase be used for gap filling reactions?
Yes, Avam® T7 DNA Polymerase can be used for gap filling reactions without strand displacement, making it suitable for various DNA manipulation techniques.
What are the storage conditions for the Avam® T7 DNA Polymerase?
The Avam® T7 DNA Polymerase should be stored at temperatures between -25°C to -15°C for optimal stability and performance.
| Quantity | 3500U |
|---|
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