Optimize DNA synthesis and modification with our Avam® T4 Gene 32 Protein, an essential tool for enhancing various molecular biology applications.
Avam® T4 Gene 32 Protein
$474.00
Purchase this item and get Product Description
Unlock the full potential of your molecular biology experiments with our Avam® T4 Gene 32 Protein, derived from the bacteriophage T4. This single-stranded DNA binding protein plays a crucial role in stabilizing single-stranded regions of DNA, making it indispensable for DNA replication, recombination, and repair processes. By binding transiently and cooperatively to single-stranded DNA sequences, the Avam® T4 Gene 32 Protein effectively coats and protects them from nucleases while facilitating interactions with DNA polymerases and other replication proteins. This enhancement of DNA synthesis-related activities extends to PCR amplification and DNA sequencing, where the protein’s presence leads to improved yields and template quality, particularly in secondary-structure-rich regions. Additionally, the Avam® T4 Gene 32 Protein stimulates the synthesis rate of T4 DNA Polymerase on primed-single-stranded substrates, resulting in a notable increase in synthesis efficiency. Supplied in a convenient solution containing glycerol, the Avam® T4 Gene 32 Protein is ready to elevate the performance of your molecular biology assays, ensuring reliable and reproducible results.
Kit Contents
● Avam® T4 Gene 32 Protein (1.0 mg at 10 mg/mL)
● Optimized Buffers
Technical Parameters
● Purity: >99%
● DNA Binding: Functional
● Single-stranded Exonuclease: <1.0% released
● Double-stranded Exonuclease: <1.0% released
● Double-stranded Endonuclease: No conversion
● E. coli DNA Contamination: <10 copies
Applications
● Enhancing DNA synthesis efficiency
● Improving PCR amplification
● Enhancing DNA sequencing in secondary-structure-rich regions
● Stimulating T4 DNA Polymerase synthesis rate
● Promoting site-specific mutagenesis
● Reducing inhibitory substances in PCR
● Eliminating extra bands during sequencing
Product FAQs
What is the role of Avam® T4 Gene 32 Protein in molecular biology assays?
Avam® T4 Gene 32 Protein stabilizes single-stranded regions of DNA, enhances DNA synthesis, and promotes interactions with DNA polymerases, thereby improving various molecular biology processes.
Can the Avam® T4 Gene 32 Protein be used for site-directed mutagenesis?
Yes, the Avam® T4 Gene 32 Protein can enhance site-specific mutagenesis efficiency by stabilizing single-stranded DNA regions and promoting DNA polymerase activity.
How should I store the Avam® T4 Gene 32 Protein?
The Avam® T4 Gene 32 Protein should be stored at -20°C to ensure stability and optimal performance.
Is the Avam® T4 Gene 32 Protein compatible with standard molecular biology protocols?
Yes, the Avam® T4 Gene 32 Protein can be seamlessly integrated into standard molecular biology protocols, including PCR amplification and DNA sequencing.
| Quantity | 2000U |
|---|
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