Avam® Stoffel Fragment DNA Polymerase is a high-performance DNA polymerase optimized for robust PCR amplification, offering enhanced thermostability, fidelity, and exonuclease-free activity.
Avam® Stoffel Fragment DNA Polymerase
$147.00
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The Avam® Stoffel Fragment DNA Polymerase is a powerful tool for molecular biology applications, delivering superior performance and reliability in PCR amplification. Engineered with precision and rigorously validated, this enables efficient and accurate DNA synthesis across a wide range of experimental conditions.
Avam® Stoffel Fragment DNA Polymerase, the core component, is a recombinant fragment derived from thermostable Taq DNA polymerase, isolated from Thermus aquaticus. This unique polymerase fragment exhibits remarkable thermostability and is devoid of exonuclease activity, ensuring faithful DNA replication even in challenging experimental setups. With a half-life of 20 minutes at 97.5°C, Avam® Stoffel Fragment DNA Polymerase amplifies fragments of up to 5 kb and leaves ‘A’ overhangs, making it ideal for various downstream applications.
The Stoffel deletion (5′-3′ exo-) enhances enzyme thermostability and fidelity, particularly beneficial for GC-rich and secondary structure templates. The increased thermal stability of Avam® Stoffel Fragment DNA Polymerase allows for denaturation temperatures as high as 98°C, facilitating superior amplification of challenging templates.
Supplied with an optimized 10x Stoffel Buffer, this provides the ideal reaction environment for Avam® Stoffel Fragment DNA Polymerase, ensuring maximum enzymatic activity and amplification efficiency. The broad MgCl2 optimum range (2.5-5 mM) reduces the need for magnesium optimization experiments, streamlining PCR protocol optimization.
Backed by stringent quality control measures, including DNase contamination evaluation through extensive PCR reactions, the Avam® Stoffel Fragment DNA Polymerase delivers consistent and reliable results, empowering researchers in diverse molecular biology applications. Experience the precision and reliability of the Avam® Stoffel Fragment DNA Polymerase and unlock new possibilities in PCR amplification, genotyping and molecular biology research with enhanced performance and efficiency.
Kit Contents
● Avam® Stoffel Fragment DNA Polymerase (500 μL at 2 U/μL)
● 10X Buffer (4 x 1.25 mL)
● 50 mM MgCl2 (4 x 1 mL)
Technical Parameters
● Optimum extension temperature: 72°C
● Purity: >99%
● Exonuclease activity: None detected
● Thermostability: Half-life of 20 minutes at 97.5°C
Applications
Avam® Stoffel Fragment DNA Polymerase finds utility in various
molecular biology applications, including:
● Amplification of short and medium-size sequences
● Diagnostic and multiplex PCR
● Genotyping, including genetic mapping using primers with arbitrary
sequences (RAPD)
● Allele-Specific Amplification PCR (ASA PCR)
● Primer extension
● Light-cycler assays due to the lack of 5’-3’ exonuclease activity
Product FAQs
Can Avam® Stoffel Fragment DNA Polymerase be used for long-range PCR amplification?
Yes, Avam® Stoffel Fragment DNA Polymerase is recommended for long-range PCR amplification, demonstrating robust performance in amplifying fragments of up to 5 kb.
Is Avam® Stoffel Fragment DNA Polymerase suitable for multiplex PCR reactions?
Yes, the Avam® Stoffel Fragment DNA Polymerase is highly compatible with multiplex PCR applications, enabling simultaneous amplification of multiple targets within the same reaction with minimal optimization.
Does Avam® Stoffel Fragment DNA Polymerase require any special reaction conditions?
Avam® Stoffel Fragment DNA Polymerase exhibits maximum activity with the provided 10x Stoffel Buffer, which ensures optimal reaction conditions. However, users may need to optimize MgCl2 concentration based on their specific experimental setup.
Is Avam® Stoffel Fragment DNA Polymerase suitable for use with challenging templates, such as GC-rich sequences?
Yes, the enhanced thermostability and fidelity of Avam® Stoffel Fragment DNA Polymerase make it particularly effective for amplifying GC-rich templates and sequences with secondary structures.
Can I use Avam Stoffel Fragment DNA Polymerase for primer extension assays?
Yes, Avam® Stoffel Fragment DNA Polymerase is suitable for primer extension assays, offering high processivity and fidelity in DNA synthesis reactions.
Is the Avam Stoffel Fragment DNA Polymerase compatible with automated PCR systems?
Yes, the Avam® Stoffel Fragment DNA Polymerase is compatible with various automated PCR systems, facilitating high-throughput applications and streamlined workflows.
| Quantity | 1000U |
|---|
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