Avam® Pyrophosphatase (E. coli) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate, thereby enhancing the efficiency of RNA and DNA synthesis reactions by removing inhibitory PPi molecules.
Avam® Pyrophosphatase (E. coli)
$322.00
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Avam® Pyrophosphatase (E. coli), facilitates the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate, catalyzing the reaction P2O7-4 + H2O → 2HPO4-2 in a magnesium-dependent manner. This enzymatic activity plays a critical role in enhancing RNA and DNA synthesis reactions by removing inhibitory PPi molecules.
The enzyme is supplied in a buffer solution containing 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol, ensuring stability and activity. Its performance is characterized by high purity (>95%) and specific activity (3500 U/mg), making it a reliable tool for various molecular biology applications.
Avam® Pyrophosphatase (E. coli) finds extensive use in in vitro transcription (IVT) or RNA synthesis reactions to mitigate the inhibitory effects of PPi. By adding 0.1-1 units per mL of enzyme to RNA synthesis reactions, researchers can optimize conditions for efficient RNA production.
The enzyme’s applications extend to DNA and RNA synthesis and modification processes, where its ability to remove PPi enhances reaction efficiency. Whether performing routine molecular biology experiments or more specialized applications, Avam® Pyrophosphatase (E. coli) serves as a valuable tool for researchers in various fields.
Kit Contents
● Avam® Pyrophosphatase (E. coli) (50 U)
● Storage buffer solution
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 19.7 kilo Daltons
● Purity: >95%
● Specific activity: 3500 U/mg
● Single-stranded exonuclease: <1% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● Facilitates DNA and RNA synthesis reactions
● Enhances in vitro transcription (IVT)
● Improves RNA synthesis efficiency
● Useful in various molecular biology assays and experiments
Product FAQs
What is the role of Avam® Pyrophosphatase (E. coli) in molecular biology?
Avam® Pyrophosphatase (E. coli) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate, thereby enhancing the efficiency of RNA and DNA synthesis reactions by removing inhibitory PPi molecules.
How should Avam® Pyrophosphatase (E. coli) be stored?
It should be stored at temperatures ranging from -25°C to -15°C for optimal stability.
What the recommended concentration of Avam® Pyrophosphatase (E. coli) for in vitro RNA synthesis reactions?
As a starting point, add 0.1-1 units per mL of Avam® Pyrophosphatase (E. coli) to identify the optimal concentration for in vitro RNA synthesis reactions.
How is the purity and activity of Avam® Pyrophosphatase (E. coli) assessed?
Purity and activity are determined through various quality control measures, including SDS-PAGE, specific activity assays, and tests for contaminating E. coli DNA.
Can Avam® Pyrophosphatase (E. coli) be used in other applications besides RNA synthesis?
Yes, it can also be used to enhance DNA synthesis reactions and in various molecular biology assays where the removal of inhibitory PPi molecules is beneficial.
| Quantity | 50U |
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