Efficient and versatile Avam® Klenow Fragment (3’→5′ exonuclease) enzyme for various molecular biology applications, offering robust DNA synthesis without proofreading activities.
Avam® Klenow Fragment (Exonuclease Free)
$507.00
Purchase this item and get Product Description
The Avam® Klenow Fragment (3′→5′ exonuclease) enzyme provides a versatile solution for various molecular biology applications, characterized by its unique features and robust performance. Derived from DNA polymerase I, this fragment exhibits a deficiency in both proofreading (3′→5′) and nick-translation (3′→5′) nuclease activities, making it ideal for specific molecular biology techniques.
The enzyme retains its 3′→5′ DNA polymerase activity, ensuring efficient DNA synthesis, while lacking proofreading and nick-translation activities, which can be advantageous for certain applications. Its moderate strand displacement activity during DNA synthesis further enhances its utility in various experimental setups.
Supplied in a convenient formulation containing 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol, the Avam® Klenow Fragment (3′→5′ exonuclease) is readily accessible for use in molecular biology laboratories. Additionally, it comes with a 10X Blue Buffer containing essential components such as NaCl, Tris-HCl, MgCl2, and DTT, ensuring optimal enzyme performance under diverse experimental conditions.
Performance-wise, the Avam® Klenow Fragment (3 exonuclease) demonstrates high purity (>90%) and specific activity (10,000 U/mg), along with minimal contaminating activities such as single-stranded and double stranded exonuclease, endonuclease, and E. coli DNA contamination, ensuring reliable and reproducible results.
The enzyme’s principle lies in its unique biochemical properties, where it lacks the 5′→3′ exonuclease activity present in DNA polymerase I, making it suitable for applications where this activity is undesirable. Instead, it retains the 5′→3′ polymerase activity and 3′→5′ exonuclease activity, essential for DNA synthesis and removal of precoding nucleotides, respectively.
Notably, the Avam® Klenow Fragment (3′→5′ exonuclease) finds widespread use in molecular biology serving as a preferred choice for DNA labeling, dA-tailing, and dT-tailing for next-generation sequencing adapter ligation. Additionally, it finds applications in dideoxy DNA sequencing, cDNA second shand synthesis, and site-directed DNA mutagenesis, underscoring its versatility and significance in various experimental setups.
Kit Contents
● Avam® Klenow Fragment (3’→5′ exonuclease) enzyme (10,000 U)
● 10X VividBlue™ (1 X 1.5 mL)
Technical Parameters
● Purity: >99%
● Specific Activity: 10,000 U/mg
● Single-stranded Exonuclease: <10% released
● Double-stranded Exonuclease: <1% released
● Double-stranded Endonuclease: No conversion
● E. coli DNA Contamination: <10 copies
● UDG Activity: <20 U/mL
Applications
● DNA labeling
● dA-tailing and dT-tailing for next-generation sequencing adapter
ligation
● Dideoxy DNA sequencing
● cDNA second strand synthesis
● Generation of single-stranded DNA probes
● Site-directed DNA mutagenesis
Product FAQs
What is the advantage of using the Avam® Kienow Fragment (3′→5′ exonuclease) enzyme?
The enzyme lacks proofreading activities, making it suitable for applications where 5′→3′ exonuclease activity is undesirable, while retaining robust DNA synthesis capabilities
Can the Avam® Klenow Fragment (3′→5′ exonuclease) enzyme be used for DNA labeling?
Yes, the enzyme is a proven choice for DNA labeling applications due to its efficient DNA polymerization activity.
What is the significance of dA-tailing and di-tailing for next-generation sequencing adapter ligation?
dA-tailing and di-tailing are essential steps in preparing DNA libraries for next-generation sequencing, and the Avam® Klenow Fragment (3′→5′ exonuclease) enzyme is commonly used for these applications.
Is the Avam® Klenow Fragment (3′→5′ exonuclease) suitable for site-directed DNA mutagenesis?
Yes, the enzyme is widely used for site-directed DNA mutagenesis experiments, facilitating the introduction of specific mutations into DNA sequences.
Can the Avam® Klenow Fragment (3′→5′ exonuclease) enzyme be stored for long periods?
Yes, when stored properly at recommended temperatures, the enzyme remains stable for extended periods, ensuring reliable performance in molecular biology experiments.
| Quantity | 10000U |
|---|
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